targocil and Autolysis

targocil has been researched along with Autolysis* in 2 studies

Other Studies

2 other study(ies) available for targocil and Autolysis

Article	Year
Exposure of Staphylococcus aureus to Targocil Blocks Translocation of the Major Autolysin Atl across the Membrane, Resulting in a Significant Decrease in Autolysis. Antimicrobial agents and chemotherapy, 2018, Volume: 62, Issue:7 Peptidoglycan (PG) and wall teichoic acid (WTA) are the major staphylococcal cell wall components, and WTA biosynthesis has recently been explored for drug development. Targocil is a novel agent that targets the TarG subunit of the WTA translocase (TarGH) that transports WTA across the membrane to the wall. Previously we showed that targocil treatment of a methicillin-susceptible Topics: Autolysis; Bacterial Translocation; Lysostaphin; Muramidase; N-Acetylmuramoyl-L-alanine Amidase; Protein Transport; Quinazolines; Staphylococcus aureus; Teichoic Acids; Triazoles	2018
An antibiotic that inhibits a late step in wall teichoic acid biosynthesis induces the cell wall stress stimulon in Staphylococcus aureus. Antimicrobial agents and chemotherapy, 2012, Volume: 56, Issue:4 Wall teichoic acids (WTAs) are phosphate-rich, sugar-based polymers attached to the cell walls of most Gram-positive bacteria. In Staphylococcus aureus, these anionic polymers regulate cell division, protect cells from osmotic stress, mediate host colonization, and mask enzymatically susceptible peptidoglycan bonds. Although WTAs are not required for survival in vitro, blocking the pathway at a late stage of synthesis is lethal. We recently discovered a novel antibiotic, targocil, that inhibits a late acting step in the WTA pathway. Its target is TarG, the transmembrane component of the ABC transporter (TarGH) that exports WTAs to the cell surface. We examined here the effects of targocil on S. aureus using transmission electron microscopy and gene expression profiling. We report that targocil treatment leads to multicellular clusters containing swollen cells displaying evidence of osmotic stress, strongly induces the cell wall stress stimulon, and reduces the expression of key virulence genes, including dltABCD and capsule genes. We conclude that WTA inhibitors that act at a late stage of the biosynthetic pathway may be useful as antibiotics, and we present evidence that they could be particularly useful in combination with beta-lactams. Topics: Anti-Bacterial Agents; Autolysis; Bacterial Proteins; beta-Lactams; Cell Wall; Culture Media; Methicillin-Resistant Staphylococcus aureus; Microarray Analysis; Microbial Sensitivity Tests; Microscopy, Electron; Microscopy, Electron, Transmission; Quinazolines; RNA, Bacterial; Staphylococcus aureus; Teichoic Acids; Transcription, Genetic; Triazoles; Virulence Factors	2012

Article

Year

Exposure of Staphylococcus aureus to Targocil Blocks Translocation of the Major Autolysin Atl across the Membrane, Resulting in a Significant Decrease in Autolysis.

Antimicrobial agents and chemotherapy, 2018, Volume: 62, Issue:7

Peptidoglycan (PG) and wall teichoic acid (WTA) are the major staphylococcal cell wall components, and WTA biosynthesis has recently been explored for drug development. Targocil is a novel agent that targets the TarG subunit of the WTA translocase (TarGH) that transports WTA across the membrane to the wall. Previously we showed that targocil treatment of a methicillin-susceptible

Topics: Autolysis; Bacterial Translocation; Lysostaphin; Muramidase; N-Acetylmuramoyl-L-alanine Amidase; Protein Transport; Quinazolines; Staphylococcus aureus; Teichoic Acids; Triazoles

2018

An antibiotic that inhibits a late step in wall teichoic acid biosynthesis induces the cell wall stress stimulon in Staphylococcus aureus.

Antimicrobial agents and chemotherapy, 2012, Volume: 56, Issue:4

Wall teichoic acids (WTAs) are phosphate-rich, sugar-based polymers attached to the cell walls of most Gram-positive bacteria. In Staphylococcus aureus, these anionic polymers regulate cell division, protect cells from osmotic stress, mediate host colonization, and mask enzymatically susceptible peptidoglycan bonds. Although WTAs are not required for survival in vitro, blocking the pathway at a late stage of synthesis is lethal. We recently discovered a novel antibiotic, targocil, that inhibits a late acting step in the WTA pathway. Its target is TarG, the transmembrane component of the ABC transporter (TarGH) that exports WTAs to the cell surface. We examined here the effects of targocil on S. aureus using transmission electron microscopy and gene expression profiling. We report that targocil treatment leads to multicellular clusters containing swollen cells displaying evidence of osmotic stress, strongly induces the cell wall stress stimulon, and reduces the expression of key virulence genes, including dltABCD and capsule genes. We conclude that WTA inhibitors that act at a late stage of the biosynthetic pathway may be useful as antibiotics, and we present evidence that they could be particularly useful in combination with beta-lactams.

Topics: Anti-Bacterial Agents; Autolysis; Bacterial Proteins; beta-Lactams; Cell Wall; Culture Media; Methicillin-Resistant Staphylococcus aureus; Microarray Analysis; Microbial Sensitivity Tests; Microscopy, Electron; Microscopy, Electron, Transmission; Quinazolines; RNA, Bacterial; Staphylococcus aureus; Teichoic Acids; Transcription, Genetic; Triazoles; Virulence Factors

2012