tannins and Wounds-and-Injuries

Topics: Animals; Female; Hydrogels; Polyethylene Glycols; Rats; Rats, Sprague-Dawley; Succinimides; Tannins; Tissue Adhesives; Wound Healing; Wounds and Injuries

Natural polymeric hydrogel featuring multifunctional properties is more attractive as wound dressing. Herein, Tannic acid (TA)-reinforced methacrylated chitosan (CSMA)/methacrylated silk fibroin (SFMA) hydrogels were fabricated by two-step method of photopolymerization and TA solution incubating treatment. The TA in hydrogels not only served as second crosslinker improving the mechanical performance of up to a 5-fold increase (5 % TA treatment) than the pristine one, but also as functional molecule that endowed the hydrogels with enhanced adhesiveness and antioxidative properties. Besides, the introduction of TA into hydrogels further improved the antimicrobial activities against both Escherichia coli (E. coli) and Staphylococcus Aureus (S. aureus), as well as the cytocompatibility on fibroblasts. Moreover, it was demonstrated that the TA-treated CSMA/SFMA hydrogels could significantly promote wound healing in a full-thickness skin defect model. Collectively, these results showed that TA-reinforced CSMA/SFMA hydrogels could be a promising candidate as wound dressing.

Topics: Adhesiveness; Animals; Anti-Bacterial Agents; Antioxidants; Bandages; Cell Proliferation; Chitosan; Cross-Linking Reagents; Escherichia coli; Fibroins; Hydrogels; Mechanical Phenomena; Methacrylates; Mice; Mice, Inbred ICR; NIH 3T3 Cells; Rheology; Staphylococcus aureus; Tannins; Ultraviolet Rays; Wound Healing; Wounds and Injuries

Wound healing is a complex process orchestrated by the regeneration of the epithelium and the remodeling of the extracellular matrix through processes like collagen deposition. Galla Rhois has been widely used in traditional Korean medicine for its various pharmacological effects, including an anticoccidial effect, however, little is known about its healing activity. The purpose of this study was to determine the effects of Galla Rhois ethanol extract (GRE) on wound healing activities, including H2O2-induced oxidative stress, cell migration, and lactate dehydrogenase (LDH) release assays using human keratinocyte (HaCaT) and dermal fibroblasts (CCD-986SK). In addition, total soluble collagen deposition and collagen gene expression for Type I and III collagen were evaluated in CCD-986SK. Total tannin and flavonoid contents for GRE were measured. GRE induced a significant increase in the number and migration of cells, along with a decrease in cell death and LDH release. In addition, it also induced the over-expression of collagen Type I and III mRNA and caused increased synthesis of total soluble collagen. The contents of total tannin and flavonoid for GRE were 55.7% ([Formula: see text][Formula: see text]mg/g) and 62.9% ([Formula: see text][Formula: see text]mg/g), respectively. The results suggest that GRE can cause accelerated wound healing by increasing cell survival, proliferation, migration, and collagen synthesis along with a potential anti-oxidant property. This evidence provides novel insight into natural therapy for tissue injury.

Topics: Animals; Cell Movement; Cell Survival; Cells, Cultured; Collagen; Epithelium; Extracellular Matrix; Fibroblasts; Free Radical Scavengers; Hemiptera; Humans; Keratinocytes; L-Lactate Dehydrogenase; Oxidative Stress; Phytotherapy; Plant Extracts; Regeneration; Rhus; Skin; Stimulation, Chemical; Tannins; Wound Healing; Wounds and Injuries

Mimosa pudica, commonly known as touch-me-not, is used in folklore medicine in arresting bleeding and in skin diseases.. There was no scientific evidence justifying the use of Mimosa pudica, therefore the present study was aimed at evaluation of wound healing activity of the plant.. In the present study the roots of Mimosa pudica were studied for wound healing activity by incorporating the methanolic and the total aqueous extract in simple ointment base B.P. in concentration of 0.5% (w/w), 1% (w/w) and 2% (w/w). Wound healing activity was studied in three types of model in rats viz. excision, incision and estimation of biochemical parameter. In case of the excision wound model wound contraction and period of epithelization was studied while in incision wound model was evaluated by determining tensile strength and hydroxyproline content in the scab.. Treatment of wound with ointment containing 2% (w/w) the methanolic and 2% (w/w) the total aqueous extract exhibited significant (P<0.001) wound healing activity. The methanolic and total aqueous extracts were analyzed for total phenols content equivalent to Gallic acid. The content of total phenols was 11% (w/w) and 17% (w/w) in methanolic and total aqueous extract respectively.. The methanolic extract exhibited good wound healing activity probably due to phenols constituents.

Topics: Administration, Cutaneous; Alkaloids; Animals; Female; Hydroxyproline; Male; Mimosa; Ointments; Phenols; Phytotherapy; Plant Extracts; Plant Roots; Rats; Skin; Tannins; Wound Healing; Wounds and Injuries