tannins and Vascular-Calcification

Vascular calcification is common in chronic kidney disease; however, the extent to which such condition can affect the renal microvasculature and the neighboring cell types is unclear. Our induced-calcification model in renal proximal tubular (PT) cells exhibited endoplasmic reticulum (ER) stress and oxidative damage, leading to apoptosis. Here, we utilized such calcification in mouse vascular smooth muscle (MOVAS-1) cells as a vascular calcification model, because it exhibited reactive oxygen species (ROS) generation, ER and oxidative stress, inflammatory, and apoptotic gene expressions. To demonstrate whether the vascular calcification condition can dictate the function of the adjacent PT cell layer, we utilized a Transwell multilayer culture system by combining those MOVAS-1 cells in the bottom chamber and polarized PT cells in the upper chamber to show the dimensional cross-signaling effect. Interestingly, calcification of MOVAS-1 cells, in this co-culture, induced H

Topics: Animals; Calcium Signaling; Cells, Cultured; Hydrogen Peroxide; Kidney Tubules, Proximal; Mice, Inbred C57BL; Muscle, Smooth, Vascular; Paracrine Communication; Protective Agents; Tannins; Vascular Calcification

Advanced glycation end products/receptor for AGEs (AGEs/RAGEs) or Toll like receptor 4 (TLR4) induce vascular smooth muscle cell (VSMC) phenotype changes in osteoblast-like cells and vascular calcification. We analyzed the effect of

Topics: Animals; Benzofurans; Diet, High-Fat; Disease Models, Animal; Male; Mice; Mice, Inbred C57BL; Muscle, Smooth, Vascular; Osteoblasts; Phenotype; Plant Extracts; Tannins; Vascular Calcification