tannins and Urinary-Bladder-Neoplasms

Topics: Animals; Breast Neoplasms; Carcinogens, Environmental; Colonic Neoplasms; Diet; Dietary Fats; Esophageal Neoplasms; Humans; Liver Neoplasms; Lung Neoplasms; Mammary Neoplasms, Experimental; Neoplasms; Pancreatic Neoplasms; Stomach Neoplasms; Tannins; Urinary Bladder Neoplasms

Intravesical instillation therapy is increasingly recognized as one of the most common clinical treatment strategies for bladder cancer. However, the antitumor efficacy of chemotherapy drugs is still limited due to their rapid clearance by periodic urination. To circumvent this issue, a drug-loaded thin film comprising the self-assembly of tannic acid (TA) and ferric ions (Fe

Topics: Administration, Intravesical; Antineoplastic Agents; Drug Liberation; Humans; Tannins; Urinary Bladder; Urinary Bladder Neoplasms

Urothelial bladder cancer is rapidly spreading across Western countries, and therapy has shown little-to-moderate effects on bladder cancer. Thus, focusing on curbing cancer incidence has become crucial. The aim of the present study was to investigate the anticancer effects of Tannic acid (TA) in human bladder cancer. UMUC3 bladder cancer cells were treated with different concentrations of TA (0-100 µM) and tested for cell viability, colony formation, and apoptosis. The involvement of the phosphoinositide-3 kinase (PI3K)/Akt pathway in the action of TA was examined. TA treatment significantly inhibited the viability and increased percentage of apoptotic cells, thereby decreasing antiapoptotic proteins (BCL2, MCL-1, and BCL-XL) expression, resulting in the Caspase-3 activation. TA treatment decreased stem cell markers expression such as SOX2, OCT4, and NANOG. Additionally, TA treatment significantly reduced the phosphorylation levels of Akt in bladder cancer cells. Our study demonstrates the growth inhibitory effects of TA in bladder cancer cells, and highlights its potential as an anticancer agent for bladder cancer.

Topics: Apoptosis; Cell Line, Tumor; Cell Proliferation; Humans; Phosphorylation; Proto-Oncogene Proteins c-akt; Tannins; Urinary Bladder Neoplasms

F344 inbred and Sprague-Dawley noninbred rats were fed a basic diet (groups 1 and 7) or a basic diet supplemented with 0.1% (later, 0.2 and 0.4%) tannin (group 2) isolated from bracken fern (Pteridium aquilinum) (BF), 33% BF (groups 3 and 6), 2% chloroform fraction of BF (group 4), or 4% tannin-free fraction of BF (group 5). The following incidences of intestinal or bladder tumors were observed: group 1, intestinal and bladder, 0/16; group 2, 0/21; group 7, 0/16; groups 4 and 5, intestinal, 7/15, bladder, 0/15; group 3, intestinal, 19/20, bladder, 12/20; and group 6, intestinal, 22/30, bladder, 15/30. The chloroform-methanol fraction prepared from urine of rats fed BF, chloroform fraction of BF, or tannin-free fraction of BF demonstrated mutagenicity for Salmonella typhimurium TA 100 but not for TA 98. No mutagenicity was detected in other prepared fractions. F344 rats in group 8 received weekly sc injections of tannin solution (0.1 g/kg body wt) for 38 weeks, and 16/20 developed palpable tumors that were malignant fibrous histiocytomas at the injection site. No tumor was present in any rat of control group 9.

Topics: Animals; Carcinogens; Female; Injections, Subcutaneous; Intestinal Neoplasms; Male; Mutagens; Plants, Edible; Plants, Toxic; Quercetin; Rats; Salmonella typhimurium; Tannins; Urinary Bladder Neoplasms

We attempted to isolate a carcinogenic substance from bracken fern (Pteridium aquilinum), a naturally occurring toxicant responsible for the production of chronic enzootic hematuria and urinary bladder cancer of cattle and carcinogenic for various target organs of several species. Hot methanol extracts of bracken fern were solubilized in water and extracted with chloroform followed by a mixture of n-butanol-butanone (1:1). That fraction was dried and triturated with ether-methanol (4:1), n-butanol, and finally absolute ethanol. The insoluble residue was dissolved in 10% aqueous methanol and passed through Dowex 1 OH-, Dowex 50 H+, or Dowex 1 OH- and then Dowex 50 H+ ion exchange resins. A condensed tannin, isolated from one ot the fractions, was identical to that isolated from bracken fern by the caffeine procedure used for the separation of tannins from other plant constituents. Three systems were used for bioassay; induction of bladder carcinoma by implantation of cholesterol pellets containing bracken fern fractions into the bladder lumens of mice; acute toxicity by ip injection of brachen fern fraction into mice; and growth inhibition of Escherichia coli. The following fractions induced significantly greater incidences of bladder carcinoma than did cholesterol pellets only: tannin, Dowex 50 H+, residue, n-butanol, and methanol. Tiliroside, a component of bracken fern fractions into the bladder lumens of mice; acute genic acid, and quercetin were not carcinogenic. Tannin was the most toxic (mean lethal dose: 0.16 mg/g) and carcinogenic. None of the carcinogenic fractions inhibited growth of E. coli.

Topics: Escherichia coli; Lethal Dose 50; Methanol; Neoplasms, Experimental; Plants; Tannins; Urinary Bladder Neoplasms