tannins and Thrombosis

Antithrombogenicity, anti-inflammation, and rapid re-endothelialization are central requirements for the long-term success of cardiovascular stents. In this work, a plant-inspired phenolic-amine chemistry strategy was developed to combine the biological functions of a plant polyphenol, tannic acid (TA), and the thrombin inhibitor bivalirudin (BVLD) for tailoring the desired multiple surface functionalities of cardiovascular stents. To realize the synergistic modification of TA and BVLD on a stent surface, an amine-bearing coating of plasma polymerized allylamine was firstly prepared on the stent surface, followed by the sequential conjugation of TA and BVLD in alkaline solution based on phenolic-amine chemistry (i.e., Michael addition reaction). TA and BVLD were successfully immobilized onto the stent surface with considerable amounts of 330 ± 12 and 930 ± 80 ng/cm

Topics: Amines; Antithrombins; Hirudins; Humans; Inflammation; Peptide Fragments; Polyphenols; Recombinant Proteins; Stents; Tannins; Thrombosis

Vascular stent interventional therapy, as a regular and effective therapy, has been widely used to treat coronary artery diseases. However, adverse events occur frequently after stent intervention, especially restenosis and late stent thrombosis. The targeted implanting site will suffer from severe atherosclerosis, which is considered as a chronic inflammatory disease. Meanwhile, with the over-expanding use of endovascular mechanical intervention, vascular injury has become an increasingly common issue. Lesions and newly induced vascular injury result in inflammatory and oxidative stress; meanwhile, activated macrophages and granulocytes generate high levels of reactive oxygen species (ROS), contributing to endothelial dysfunction and neointima hyperplasia. Therefore, attenuating oxidative stress and reducing ROS generation in the inflammatory response represent reasonable strategies to inhibit intimal hyperplasia and restenosis. Herein, we have developed a multifunctional surface for the MgZnYNd alloy with tannic acid (TA) coating, and the pH dependence of the coating deposition is also demonstrated. The phenolic hydroxyl groups on the coatings endow the modified surface with excellent antioxidant functions. We found that the coating can be recycled, and the scavenging activity hardly weakened within five cycles. Also, the TA coating has a promising strong antioxidant activity as it shows a radical scavenging activity over 80% in long term. Moreover, the TA coating possesses platelet-repellent capability. No significant inflammatory response was observed for the TA modified sample in the rat subcutaneous implantation test. Combining these performances, we envision that the vascular stent modified with TA coating can have great potential in various applications by virtue of its simplicity and effectiveness.

Topics: Alloys; Animals; Antioxidants; Biphenyl Compounds; Blood Platelets; Cell Proliferation; Coronary Restenosis; Fluorides; Human Umbilical Vein Endothelial Cells; Humans; Hydrogen-Ion Concentration; Inflammation; Neodymium; Oxidative Stress; Picrates; Platelet Adhesiveness; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species; Stents; Tannins; Thrombosis; Zinc

Topics: Adenosine Diphosphate; Aminocaproates; Animals; Aprotinin; Benzopyrans; Carotid Artery Thrombosis; Epinephrine; Female; Ketones; Rats; Serotonin; Tannins; Thrombin; Thrombosis

Topics: Animals; Blood; Blood Cell Count; Blood Platelets; Blood Vessels; Buffers; Citrates; Collagen; Densitometry; Disease Models, Animal; Dose-Response Relationship, Drug; Endothelium; Hematocrit; Hydrogen-Ion Concentration; Hypoxia; Lactates; Platelet Adhesiveness; Rats; Tannins; Thrombosis

Topics: Animals; Hemorrhagic Disorders; Norepinephrine; Rats; Shwartzman Phenomenon; Skin Diseases; Tannins; Thorium Dioxide; Thrombosis

Topics: Agar; Animals; Carrageenan; Cold Temperature; Epinephrine; Hemorrhagic Disorders; Indium; Rats; Tannins; Thorium Dioxide; Thrombosis