tannins and Plague

A stable hemagglutinating antigen for detection of fraction I (FR-I) antibody of Yersinia pestis (Pasteurella pestis) is described. The antigen was prepared by sensitizing tanned, pyruvaldehyde-treated sheep erythrocytes (PAT SRBC) with FR-I antigen. Preliminary standardization by titration of each lot of FR-I was required to minimize the effect of molecular heterogeneity of specific FR-I antigen and to eliminate nonspecific reactions caused by the presence of a minor antigenic contaminant. In tests with sera from rabbits, dogs, and humans, FR-I PAT SRBC were as reactive as the previously employed standard antigen, FR-I-sensitized tanned erythrocytes. Fluid suspensions of FR-I PAT SRBC stored at 4 C for 3 months, or lyophilized preparations stored at ambient temperature for 6 months, showed no loss in antigenic activity.

Topics: Aldehydes; Animals; Antibodies; Antigen-Antibody Reactions; Antigens; Antigens, Bacterial; Blood Preservation; Dogs; Erythrocytes; Freeze Drying; Hemagglutination Tests; Humans; Immune Sera; Immunochemistry; Indicators and Reagents; Plague; Rabbits; Sheep; Tannins; Time Factors; Yersinia pestis

Topics: Antigens; Erythrocytes; Freeze Drying; gamma-Globulins; Hemagglutination Tests; Humans; Methods; Plague; Tannins