tannins and Multiple-Myeloma

Multiple myeloma (MM) is a clonal B-cell malignancy characterized by an accumulation of clonal plasma cells (PC) in the bone marrow (BM) leading to bone destruction and BM failure. Despite recent advances in pharmacological therapy, MM remains a largely incurable pathology. Therefore, novel effective and less toxic agents are urgently necessary. In the last few years, pomegranate has been studied for its potential therapeutic properties including treatment and prevention of cancer. Pomegranate juice (PGJ) contains a number of potential active compounds including organic acids, vitamins, sugars, and phenolic components that are all responsible of the pro-apoptotic effects observed in tumor cell line. The aim of present investigation is to assess the antiproliferative and antiangiogenic potential of the PGJ in human multiple myeloma cell lines. Our data demonstrate the anti-proliferative potential of PGJ in MM cells; its ability to induce G0/G1 cell cycle block and its anti-angiogenic effects. Interestingly, sequential combination of bortezomib/PGJ improved the cytotoxic effect of the proteosome inhibitor. We investigated the effect of PGJ on angiogenesis and cell migration/invasion. Interestingly, we observed an inhibitory effect on the tube formation, microvessel outgrowth aorting ring and decreased cell migration and invasion as showed by wound-healing and transwell assays, respectively. Analysis of angiogenic genes expression in endothelial cells confirmed the anti-angiogenic properties of pomegranate. Therefore, PGJ administration could represent a good tool in order to identify novel therapeutic strategies for MM treatment, exploiting its anti-proliferative and anti-angiogenic effects. Finally, the present research supports the evidence that PGJ could play a key role of a future therapeutic approach for treatment of MM in order to optimize the pharmacological effect of bortezomib, especially as adjuvant after treatment.

Topics: Angiogenesis Inhibitors; Antineoplastic Agents, Phytogenic; Antioxidants; Beverages; Cell Line, Tumor; Cell Movement; Cell Proliferation; Cell Survival; Flavonoids; Fruit; Humans; Lythraceae; Multiple Myeloma; Neoplasm Invasiveness; Phytotherapy; Tannins; Vascular Endothelial Growth Factor A

Reactions of murine myeloma cells with infectious mononucleosis sera were studied by means of cytolysis in agarose gel. Of 75 sera tested, 30 lysed IgM myeloma cells, MOPC-104E. The antibodies responsible for the lysis of the myeloma cells were shown to be different from Paul-Bunnell antibodies and other antibodies found in infectious mononucleosis sera. Three types of antibodies acting upon the myeloma cells were identified serologically on the basis of absorption experiments with bovine erythrocytes, theta-positive murine lymphoma cells and guinea pig kidney cells. Antibodies of the first group could be absorbed with none of these antigens, antibodies of the second group could be absorbed only with lymphoma cells, and antibodies of the third group could be absorbed with any of these three antigens. Evidence was presented that the antibodies under study combine with antigenic cell membrane components of a subpopulation of IgM-producing murine B cells.

Topics: Absorption; Animals; Antibody-Producing Cells; Complement System Proteins; Cytotoxicity Tests, Immunologic; Erythrocytes; Fluorescent Antibody Technique; Hemagglutination Tests; Hemolytic Plaque Technique; Humans; Immune Sera; Immunodiffusion; Immunoglobulin M; Infectious Mononucleosis; Leukemia; Lymphocyte Depletion; Lymphoma; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Inbred DBA; Multiple Myeloma; Rabbits; Rodent Diseases; Tannins

Topics: Adolescent; Adult; Alcoholism; Antibodies; Antibody Formation; Antigens; Bronchitis; Child; Child, Preschool; Chromatography, Gel; Chronic Disease; Coronary Disease; Diabetes Mellitus; Erythrocytes; Female; Flagella; Hemagglutination Tests; Humans; Immunization, Secondary; Infant; Isoantibodies; Male; Mercaptoethanol; Multiple Myeloma; Peptic Ulcer; Postgastrectomy Syndromes; Salmonella; Tannins

Topics: Animals; Arthritis, Rheumatoid; Complement Inactivator Proteins; Erythrocytes; Guinea Pigs; Hemagglutination Tests; Hemolysin Proteins; Hot Temperature; Humans; Immune Sera; Immunodiffusion; Mercaptoethanol; Multiple Myeloma; Protein Denaturation; Rabbits; Rheumatoid Factor; Sheep; Tannins; Waldenstrom Macroglobulinemia

Topics: Antibodies; Blood Group Antigens; Caffeine; Electrophoresis; Erythroblastosis, Fetal; Female; Humans; Immunoelectrophoresis; Methods; Multiple Myeloma; Pregnancy; Saliva; Specimen Handling; Tannins; Waldenstrom Macroglobulinemia