tannins and Liver-Cirrhosis

We investigated the ameliorative effects and potential mechanisms of tannic acid (TA) in carbon tetrachloride (CCl4)-intoxicated mice and hepatic stellate cells (HSCs). Liver fibrosis was observed in CCl4 (800 ml/kg)-induced mice, and high viability was observed in CCl4 (10 mM)-intoxicated HSCs. Pre-treatment of mice with TA (25 or 50 g/kg/day) significantly ameliorated hepatic morphology and coefficient values and reduced the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), the concentrations of malondialdehyde (MDA) and serum levels of endothelin-1 (ET-1). In addition, TA increased the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and endothelial nitric oxide synthase (eNOS) and the serum level of NO. Moreover, TA reduced the expression of angiotensin II receptor-1 (ATR-1), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), caspase-3, c-fos, c-jun, the ratio of Bax/bcl-2, tissue inhibitor of metalloproteinase-1 (TIMP-1) and TA increased matrix metal proteinase-9 (MMP-9), matrix metalloproteinase-1 (MMP-1). Furthermore, TA (0.01 μM, 0.1 μM or 1 μM) decreased the TIMP-1/MMP-1 ratio and reduced the viability of HSCs. These results indicated that TA exerts significant liver-protective effects in mice with CCl4-induced liver fibrosis. The potential mechanism may rely on the inhibition of collagen accumulation, oxidative stress, inflammation and apoptosis.

Topics: Alanine Transaminase; Animals; Apoptosis; Aspartate Aminotransferases; Carbon Tetrachloride; Cell Survival; Cells, Cultured; Collagen; Humans; Inflammation; Liver; Liver Cirrhosis; Male; Matrix Metalloproteinase 1; Matrix Metalloproteinase 9; Mice; Oxidative Stress; Superoxide Dismutase; Tannins

Topics: Alkaline Phosphatase; Aspartate Aminotransferases; Barium Sulfate; Bilirubin; Carcinoma; Enema; Humans; Liver; Liver Cirrhosis; Liver Function Tests; Pancreatic Neoplasms; Serum Albumin; Tannins

Topics: Animals; Antibody Formation; Autopsy; Biopsy; Carcinoma, Hepatocellular; Cell Transformation, Neoplastic; Cells, Cultured; Chromosome Aberrations; Cytopathogenic Effect, Viral; Duodenal Ulcer; Fluorescent Antibody Technique; Hepatitis B Antigens; Hepatitis B virus; Humans; Immunodiffusion; Lectins; Liver Abscess, Amebic; Liver Cirrhosis; Liver Neoplasms; Lymphocyte Activation; Neoplasm Transplantation; Rats; Tannins

Topics: Animals; Liver; Liver Cirrhosis; Liver Neoplasms; Mice; Tannins

Topics: Coombs Test; Erythrocytes; Humans; Immune Sera; Liver Cirrhosis; Tannins

The administration to albino rats of tannic acid as a retention enema (in doses of 0.2 g./kg. body weight and over) prolonged the duration of anesthesia induced by thiopental given immediately before, or 72 hours after, the tannic acid. This dose of tannic acid corresponds, on the basis of body weight, to a radiodiagnostic enema of 2 1. of 0.25% tannic acid in barium sulfate suspension given to a child weighing 25 kg. By excluding certain hypothermic effects of tannic acid, it was concluded that thiopental potentiation was probably due to impairment by the tannic acid of the liver's ability to detoxify the barbiturate. The results suggest that a drug which is detoxified in the liver should be administered three to five days after a tannic acid-barium sulfate radiodiagnostic enema only with considerable caution.

Topics: Anesthesia; Animals; Contrast Media; Liver Cirrhosis; Radiography; Rats; Tannins; Thiopental

Topics: Barium Sulfate; Chemical and Drug Induced Liver Injury; Child; Enema; Geriatrics; Hepatitis; Hepatitis A; Humans; Liver Cirrhosis; Radiology; Tannins; Toxicology

Topics: Blood Platelets; Erythrocytes; Fibrinolysis; Hemagglutination; Hemagglutination Tests; Humans; Liver Cirrhosis; Tannins

Topics: Animals; Liver Cirrhosis; Liver Cirrhosis, Experimental; Tannins