tannins and Heart-Diseases

It remains a great challenge for targeted therapy of heart diseases. To achieve desirable heart targeting, we developed a polyphenol-assisted nanoprecipitation/self-assembly approach for facile engineering of functional nanoparticles. Three different materials were employed as representative carriers, while gallic acid, catechin, epigallocatechin gallate, and tannic acid (TA) served as typical polyphenols with varied numbers of phenolic hydroxyl groups. By optimizing different parameters, such as polyphenol types and the weight ratio of carrier materials and polyphenols, well-defined nanoparticles with excellent physicochemical properties can be easily prepared. Regardless of various carrier materials, TA-derived nanoparticles showed potent reactive oxygen species-scavenging activity, especially nanoparticles produced from a cyclodextrin-derived bioactive material (TPCD). By internalization into cardiomyocytes, TPCD/TA nanoparticles (defined as TPTN) effectively protected cells from hypoxic-ischemic injury. After intravenous injection, TPTN considerably accumulated in the injured heart in two murine models of ventricular fibrillation cardiac arrest in rats and myocardial hypertrophy in mice. Correspondingly, intravenously delivered TPTN afforded excellent therapeutic effects in both heart diseases. Preliminary experiments also revealed good safety of TPTN. These results substantiated that TPTN is a promising nanotherapy for targeted treatment of heart diseases, while polyphenol-assisted self-assembly is a facile but robust strategy to develop heart-targeting delivery systems.

Topics: Animals; Heart Diseases; Mice; Nanoparticles; Polyphenols; Rats; Reactive Oxygen Species; Tannins

Doxorubicin, an anthracycline antibiotic, is widely used in the treatment of various solid tumors including breast cancer. However, its use is limited due to a variety of toxicities including cardiotoxicity. The present study aimed to evaluate the effect of tannic acid, a PARG/PARP inhibitor and an antioxidant, on doxorubicin-induced cardiotoxicity in H9c2 embryonic rat heart myoblasts and its anti-cancer activity in MDA-MB-231 human breast cancer cells as well as in DMBA-induced mammary tumor animals. Doxorubicin-induced cardiotoxicity was assessed by measurement of heart weight, plasma LDH level and histopathology. Bcl-2, Bax, PARP-1 and p53 expression were examined by western blotting. Our results show that tannic acid prevents activation of PARP-1, reduces Bax and increases Bcl-2 expression in H9c2 cells, thus, preventing doxorubicin-induced cell death. Further, it reduces the cell viability of MDA-MB-231 breast cancer cells, increases p53 expression in mammary tumors and shows maximum tumor volume reduction, suggesting that tannic acid potentiates the anti-cancer activity of doxorubicin. To the best of our knowledge, this is the first report which shows that tannic acid ameliorates doxorubicin-induced cardiotoxicity and potentiates its anti-cancer activity both in vitro (H9c2 and MDA-MB-231 cells) as well as in in vivo model of DMBA-induced mammary tumor animals.

Topics: Animals; Antibiotics, Antineoplastic; Antioxidants; Breast Neoplasms; Cell Line, Tumor; Cell Survival; Cells, Cultured; Doxorubicin; Drug Synergism; Female; Gene Expression Regulation; Heart Diseases; Humans; Mammary Neoplasms, Experimental; Myoblasts, Cardiac; Rats; Rats, Sprague-Dawley; Tannins; Tumor Suppressor Protein p53

Topics: Antibodies; Antigens; Aorta; Aortic Diseases; Collagen Diseases; Complement Fixation Tests; Creatine Kinase; Endocrine System Diseases; Esophageal Diseases; Fluorescent Antibody Technique; Heart Diseases; Hemagglutination Tests; Hematologic Diseases; Humans; Immunoglobulins; Inflammation; Kidney Diseases; Liver Diseases; Lung Diseases; Microbial Collagenase; Nervous System Diseases; Precipitin Tests; Streptococcus; Tannins; Vascular Diseases

Topics: Adolescent; Antibodies; Antibodies, Anti-Idiotypic; Arthritis; Child; Geriatrics; Heart Diseases; Hemagglutination; Humans; Nephritis; Periodontal Diseases; Rheumatic Fever; Rheumatic Heart Disease; Scarlet Fever; Streptococcal Infections; Streptococcus; Tannins; Thyroid Diseases