tannins and Gastritis

In the present study we chemically profiled tannin-enriched extracts from strawberries and tested their biological properties in a cell model of gastric inflammation. The chemical and biological features of strawberry tannins after in vitro simulated gastric digestion were investigated as well. The anti-inflammatory activities of pure strawberry tannins were assayed to get mechanistic insights. Tannin-enriched extracts from strawberries inhibit IL-8 secretion in TNFα-treated human gastric epithelial cells by dampening the NF-κB signaling. In vitro simulated gastric digestion slightly affected the chemical composition and the biological properties of strawberry tannins. By using pure compounds, we found that casuarictin may act as a pure NF-κB inhibitor while agrimoniin inhibits IL-8 secretion also acting on other biological targets; in our system procyanidin B1 prevents the TNFα-induced effects without interfering with the NF-κB pathway. We conclude that strawberry tannins, even after in vitro simulated gastric digestion, exert anti-inflammatory activities at nutritionally relevant concentrations.

Topics: Anti-Inflammatory Agents; Cell Line, Tumor; Dose-Response Relationship, Drug; Epithelial Cells; Fragaria; Gastric Mucosa; Gastritis; Humans; Interleukin-8; NF-kappa B; Phytotherapy; Plant Extracts; Plants, Medicinal; Promoter Regions, Genetic; Signal Transduction; Tannins; Transfection; Tumor Necrosis Factor-alpha

The inhibitory effects of a standarized apple peel polyphenol-rich extract (APPE) against Helicobacter pylori infection and vacuolating bacterial toxin (VacA) induced vacuolation were investigated. Apple peel polyphenols significantly prevented vacuolation in HeLa cells with an IC(50) value of 390 microg of gallic acid equivalents (GAE)/mL. APPE also displayed an in vitro antiadhesive effect against H. pylori. A significant inhibition was observed with a 20-60% reduction of H. pylori attachment at concentrations between 0.250 and 5 mg of GAE/mL. In a short-term infection model (C57BL6/J mice), two levels of APPE doses (150 and 300 mg/kg/day) showed an inhibitory effect on H. pylori attachment. Orally administered apple peel polyphenols also showed an anti-inflammatory effect on H. pylori-associated gastritis, lowering malondialdehyde levels and gastritis scores.

Topics: Administration, Oral; Animals; Chlorogenic Acid; Flavonoids; Gastritis; HeLa Cells; Helicobacter Infections; Helicobacter pylori; Humans; Malus; Mice; Mice, Inbred C57BL; Tannins

Helicobacter pylori colonizes the human gastric mucosa, causing inflammation that leads to atrophic gastritis, and it can cause peptic ulcer and gastric cancer. We show that polyphenol administration to mice experimentally infected by H. pylori or treated with VacA toxin can limit gastric epithelium damage, an effect that may be linked to VacA inhibition.

Topics: Animals; Bacterial Proteins; Colony Count, Microbial; Flavonoids; Gastric Mucosa; Gastritis; Helicobacter Infections; Helicobacter pylori; Humans; Mice; Mice, Inbred BALB C; Phenols; Polyphenols; Specific Pathogen-Free Organisms; Tannins

Topics: Biliary Tract Diseases; Duodenal Ulcer; Gastric Acidity Determination; Gastric Juice; Gastritis; Glycoproteins; Humans; Hydrochloric Acid; Mucoproteins; Neuraminic Acids; Salicylates; Stomach Ulcer; Tannins

Topics: Animals; Gastritis; Gastrointestinal Diseases; Phenols; Quercus; Stomach Ulcer; Tannins; Trees