tannins and Anaphylaxis

Recent Australian and international legislation requires labeling of wines made by using the potentially allergenic food proteins casein, milk, egg white, or isinglass (fish-derived) where "there is a detectable residual processing aid." We investigated whether wines fined using these proteins or non-grape-derived tannins (tree-nut derived) can provoke significant clinical allergic reactions (anaphylaxis) in patients with confirmed immunoglobulin E-mediated relevant food allergy.. A double-blind, placebo-controlled trial was performed to determine whether allergic reactions followed consumption of Australian commercial wines fined using one or more of the legislation-targeted food proteins. In addition, allergenicity of a larger panel of these wines was evaluated by blood basophil activation.. No anaphylaxis was induced by wine consumption. Three mild clinical reactions to protein-fined wine and two mild reactions to unfined wine occurred, but there was no statistically significant difference in reaction parameters between subject groups or between processing aids. No pattern of basophil activation correlated with wine type, processing aid, or subject group.. Wines fined with egg white, isinglass, or non-grape-derived tannins present an extremely low risk of anaphylaxis to fish-, egg-, or peanut-allergic consumers. Although consumption of milk protein-fined wine did not induce anaphylaxis, there were insufficient subjects to determine statistically whether wines fined with milk proteins present a risk to the very rare milk-allergic consumers. In summary, the observed lack of anaphylaxis and basophil activation induced by wines made using the legislation-targeted food proteins according to good manufacturing practice suggests negligible residual food allergens in these wines.

Topics: Adult; Allergens; Anaphylaxis; Arachis; Basophils; Caseins; Double-Blind Method; Female; Food Contamination; Food Handling; Food Hypersensitivity; Gelatin; Humans; Male; Middle Aged; Ovalbumin; Tannins; Wine; Young Adult

Natural medicine injections have been widely used in clinics, while adverse reaction reports also have increased rapidly in recent years. To examine the anaphylactoid constituents of natural medicine injections, RBL-2H3 cell degranulation and human serum complement activation models were used to screen the anaphylactoid constituents, and the BN rat model was used to explore the anaphylactoid mechanism of these constituents. The result of an in vitro study showed that the individual compounds of natural medicine injections (chlorogenic acid, hyodeoxycholic acid, cholalic acid, ginkgolic acid, phillyrin, schisandrin B, schisandrin A, puerarin, and tanshinone IIA) and polysaccharide could not induce RBL-2H3 to release histamine and β-hexosaminidase, while proteins Tween-80 and tannic acid were the main anaphylactoid constituents in the natural medicine injections. The in vivo study also indicated that >10kDa molecules (proteins) activated classical complement pathways through direct stimulation to cause an anaphylactoid reaction. Tween-80 activated direct stimulation and coagulation pathways through classical and alternative pathways; tannic acid induced anaphylactoid reaction through co-activation of the kallikrein-kinin system, coagulation, integrated, classical and alternative complement pathways. This is the first study to evaluate the anaphylactoid constituents systematically through in vitro and in vivo study. And tannic acid, >10kDa molecules (proteins), and injection additives such as Tween-80 are the main anaphylactoid constituents of natural medicine injections.

Topics: Anaphylaxis; Animals; Basophils; Cell Degranulation; Cell Line; Complement Activation; Drug Compounding; Drugs, Chinese Herbal; Histamine; Humans; Male; Polysorbates; Rats; Rats, Inbred BN; Tannins

Topics: Aluminum; Anaphylaxis; Animals; Antibodies; Antibody Formation; Cattle; Chemical Precipitation; Cold Temperature; Erythrocytes; Female; Hemagglutination Tests; Immunization, Secondary; Male; Mercaptoethanol; Mice; Pertussis Vaccine; Serum Albumin, Bovine; Sheep; Tannins; Time Factors

Topics: Adult; Anaphylaxis; Angioedema; Anhydrides; Animals; Antibodies; Antibody Formation; Antibody Specificity; Antigens; Aspirin; Carrier Proteins; Cattle; Drug Contamination; Drug Eruptions; Drug Hypersensitivity; Erythrocytes; Female; gamma-Globulins; Guinea Pigs; Haptens; Hemagglutination Tests; Humans; Hypersensitivity, Immediate; Immunodiffusion; Lysine; Male; Middle Aged; Ovalbumin; Passive Cutaneous Anaphylaxis; Serum Albumin; Skin Tests; Tannins; Urticaria

Topics: Anaphylaxis; Animals; Antibodies; Antigens; Disease Models, Animal; Dogs; Erythrocytes; Freund's Adjuvant; Hemagglutination Tests; Immunization; Immunization, Secondary; Pollen; Sheep; Skin Tests; Tannins

Topics: Anaphylaxis; Hypersensitivity; Immune System Diseases; Intestines; Tannins