sybr-green-i and Stomach-Neoplasms

sybr-green-i has been researched along with Stomach-Neoplasms* in 2 studies

Other Studies

2 other study(ies) available for sybr-green-i and Stomach-Neoplasms

Article	Year
A simple fluorescence aptasensor for gastric cancer exosome detection based on branched rolling circle amplification. Nanoscale, 2020, Jan-28, Volume: 12, Issue:4 Exosomes are membrane nanovesicles carrying molecular information that may reflect the biological and genetic characteristics of their parent cells. Numerous studies have demonstrated the potential of exosomes as noninvasive cancer biomarkers. Hence, specific detection of cancer cell-derived exosomes is of significant importance. Here, we developed a fluorescence assay for the determination of gastric cancer exosomes based on branched rolling circle amplification (BRCA) and an aptamer to target specific exosomes. The designed padlock probe was cyclized after incubation with an aptamer binding with the target exosome. BRCA was triggered by adding a second primer and the resulting long tandem double-stranded DNA product was detected using SYBR Green I as the fluorescent dye. This method demonstrated a high specificity for target exosomes with a detection limit of 4.27 × 10 Topics: Aptamers, Nucleotide; Benzothiazoles; Biosensing Techniques; Cell Line, Tumor; Diamines; DNA; Exosomes; Fluorescent Dyes; Humans; Limit of Detection; Nucleic Acid Amplification Techniques; Organic Chemicals; Quinolines; Sensitivity and Specificity; Spectrometry, Fluorescence; Stomach Neoplasms; Temperature	2020
Quantitative detection of methylated SOCS-1 , a tumor suppressor gene, by a modified protocol of quantitative real time methylation-specific PCR using SYBR green and its use in early gastric cancer detection. Biotechnology letters, 2004, Volume: 26, Issue:16 Although methylation-specific PCR (MSP) is a sensitive technique in the detection of DNA hypermethylation, it is not quantitative. Here we described a modified PCR protocol to quantify methylated SOCS-1 gene by real time MSP using SYBR green, which involves an additional PCR step after the 72 degrees C extension step. This modified protocol is also useful in the quantitative detection of methylated SOCS-1 gene in serum samples of gastric cancer patients. Topics: Adult; Benzothiazoles; Biomarkers, Tumor; Diamines; DNA Methylation; Female; Genes, Tumor Suppressor; Genetic Predisposition to Disease; Genetic Testing; Humans; Intracellular Signaling Peptides and Proteins; Male; Methylation; Middle Aged; Organic Chemicals; Polymerase Chain Reaction; Quinolines; Repressor Proteins; Reproducibility of Results; Sensitivity and Specificity; Stomach Neoplasms; Suppressor of Cytokine Signaling 1 Protein; Suppressor of Cytokine Signaling Proteins	2004

Article

Year

A simple fluorescence aptasensor for gastric cancer exosome detection based on branched rolling circle amplification.

Nanoscale, 2020, Jan-28, Volume: 12, Issue:4

Exosomes are membrane nanovesicles carrying molecular information that may reflect the biological and genetic characteristics of their parent cells. Numerous studies have demonstrated the potential of exosomes as noninvasive cancer biomarkers. Hence, specific detection of cancer cell-derived exosomes is of significant importance. Here, we developed a fluorescence assay for the determination of gastric cancer exosomes based on branched rolling circle amplification (BRCA) and an aptamer to target specific exosomes. The designed padlock probe was cyclized after incubation with an aptamer binding with the target exosome. BRCA was triggered by adding a second primer and the resulting long tandem double-stranded DNA product was detected using SYBR Green I as the fluorescent dye. This method demonstrated a high specificity for target exosomes with a detection limit of 4.27 × 10

Topics: Aptamers, Nucleotide; Benzothiazoles; Biosensing Techniques; Cell Line, Tumor; Diamines; DNA; Exosomes; Fluorescent Dyes; Humans; Limit of Detection; Nucleic Acid Amplification Techniques; Organic Chemicals; Quinolines; Sensitivity and Specificity; Spectrometry, Fluorescence; Stomach Neoplasms; Temperature

2020

Quantitative detection of methylated SOCS-1 , a tumor suppressor gene, by a modified protocol of quantitative real time methylation-specific PCR using SYBR green and its use in early gastric cancer detection.

Biotechnology letters, 2004, Volume: 26, Issue:16

Although methylation-specific PCR (MSP) is a sensitive technique in the detection of DNA hypermethylation, it is not quantitative. Here we described a modified PCR protocol to quantify methylated SOCS-1 gene by real time MSP using SYBR green, which involves an additional PCR step after the 72 degrees C extension step. This modified protocol is also useful in the quantitative detection of methylated SOCS-1 gene in serum samples of gastric cancer patients.

Topics: Adult; Benzothiazoles; Biomarkers, Tumor; Diamines; DNA Methylation; Female; Genes, Tumor Suppressor; Genetic Predisposition to Disease; Genetic Testing; Humans; Intracellular Signaling Peptides and Proteins; Male; Methylation; Middle Aged; Organic Chemicals; Polymerase Chain Reaction; Quinolines; Repressor Proteins; Reproducibility of Results; Sensitivity and Specificity; Stomach Neoplasms; Suppressor of Cytokine Signaling 1 Protein; Suppressor of Cytokine Signaling Proteins

2004