sybr-green-i and Pneumonia--Viral

In March 2020, WHO declared a pandemic state due to SARS-CoV-2 having spread. TaqMan-based real-time RT-qPCR is currently the gold standard for COVID-19 diagnosis. However, it is a high-cost assay, inaccessible for the majority of laboratories around the world, making it difficult to diagnose on a large scale. The objective of this study was to standardize lower cost molecular methods for SARS-CoV-2 identification. E gene primers previously determined for TaqMan assays by Colman et al. (2020) were adapted in SYBR Green assay and RT-PCR conventional. The cross-reactivity test was performed with 17 positive samples for other respiratory viruses, and the sensibility test was performed with 8 dilutions (10 based) of SARS-CoV-2 isolated and 63 SARS-CoV-2-positive samples. The SYBR Green assays and conventional RT-PCR have not shown amplification of the 17 respiratory samples positives for other viruses. The SYBR Green-based assay was able to detect all 8 dilutions of the isolate. The conventional PCR detected until 10

Topics: Adolescent; Adult; Animals; Benzothiazoles; Betacoronavirus; Child; Chlorocebus aethiops; Coronavirus Infections; COVID-19; Cross Reactions; Diamines; Fluorescent Dyes; Humans; Middle Aged; Nasopharynx; Organic Chemicals; Oropharynx; Pandemics; Pneumonia, Viral; Quinolines; Real-Time Polymerase Chain Reaction; RNA, Viral; SARS-CoV-2; Sensitivity and Specificity; Vero Cells; Young Adult