sybr-green-i and Lymphocytosis

sybr-green-i has been researched along with Lymphocytosis* in 1 studies

Other Studies

1 other study(ies) available for sybr-green-i and Lymphocytosis

Article	Year
The rapid quantitative analysis of bovine cytokine genes by real-time RT-PCR. Veterinary microbiology, 2003, Jul-30, Volume: 94, Issue:4 For a practical need, fast and efficient methods to quantify mRNA expression are expecting. By using real-time reverse transcription polymerase chain reaction (RT-PCR) with the double-stranded DNA-binding dye SYBR Green I as a novel method, cytokine profiles (IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, IL-10, IL-12p40 and IFN-gamma) were analyzed in peripheral blood mononuclear cells (PBMCs) from bovine leukemia virus (BLV)-infected animals. In aleukemic cattle, IFN-gamma and IL-12p40 mRNA expression was significantly increased compared to those in cattle with persistent lymphocytosis. The similar results were obtained in the case of sheep experimentally infected with BLV. Real-time quantitative PCR technique is an applicable technique for analysis of cytokine profiles in field. Topics: Animals; Benzothiazoles; Cattle; Cytokines; Diamines; Enzootic Bovine Leukosis; Fluorescent Dyes; Leukemia Virus, Bovine; Lymphocytosis; Organic Chemicals; Quinolines; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Sheep	2003

Article

Year

The rapid quantitative analysis of bovine cytokine genes by real-time RT-PCR.

Veterinary microbiology, 2003, Jul-30, Volume: 94, Issue:4

For a practical need, fast and efficient methods to quantify mRNA expression are expecting. By using real-time reverse transcription polymerase chain reaction (RT-PCR) with the double-stranded DNA-binding dye SYBR Green I as a novel method, cytokine profiles (IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, IL-10, IL-12p40 and IFN-gamma) were analyzed in peripheral blood mononuclear cells (PBMCs) from bovine leukemia virus (BLV)-infected animals. In aleukemic cattle, IFN-gamma and IL-12p40 mRNA expression was significantly increased compared to those in cattle with persistent lymphocytosis. The similar results were obtained in the case of sheep experimentally infected with BLV. Real-time quantitative PCR technique is an applicable technique for analysis of cytokine profiles in field.

Topics: Animals; Benzothiazoles; Cattle; Cytokines; Diamines; Enzootic Bovine Leukosis; Fluorescent Dyes; Leukemia Virus, Bovine; Lymphocytosis; Organic Chemicals; Quinolines; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Sheep

2003