sybr-green-i and Haemonchiasis

Haemonchus contortus is one of the most important gastrointestinal nematodes (GINs) infecting sheep, goats, and cattle worldwide. We developed a SYBR Green real-time PCR (qPCR) assay for detection and quantification of H. contortus by using specific primers based on a conserved region of the mitochondrial cytochrome oxidase subunit I (mt-COI) gene, and evaluated this technique in the detection of H. contortus infections in cattle in Central Anatolia Region of Turkey. The newly developed qPCR assay successfully discriminated H. contortus from other GIN species infecting cattle in the specificity evaluations, with a specific melt peak of 77.5 °C. Our results revealed the efficient amplification of the proposed target COI region within the range of plasmid copies, from 2 × 10

Topics: Animal Husbandry; Animals; Benzothiazoles; Cattle; Cattle Diseases; Diamines; Electron Transport Complex IV; Haemonchiasis; Haemonchus; Organic Chemicals; Quinolines; Real-Time Polymerase Chain Reaction; Species Specificity; Turkey