sybr-green-i and Ectromelia--Infectious

Ectromelia virus (ECTV) is the causative agent of mousepox, which has devastating effects in laboratory-mouse colonies and causes economic loss in biomedical research. More importantly, ECTV has been extensively used as an excellent model for studies of the pathogenesis and immunobiology of human smallpox. A rapid and sensitive SYBR Green I-based real-time PCR assay was developed and used for the detection and quantitation of orthopoxvirus by using ECTV in this study. Primers targeted to the highly conserved region of major core protein P4b gene of orthopoxvirus were designed and the standard plasmid was constructed. This assay was able to detect a minimum of 10 copies of standard DNA and 5 TCID

Topics: Animals; Benzothiazoles; Chlorocebus aethiops; Diamines; Ectromelia virus; Ectromelia, Infectious; Limit of Detection; Male; Mice, Inbred C57BL; Organic Chemicals; Orthopoxvirus; Quinolines; Real-Time Polymerase Chain Reaction; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Vero Cells