sybr-green-i and Asthma

sybr-green-i has been researched along with Asthma* in 1 studies

Other Studies

1 other study(ies) available for sybr-green-i and Asthma

ArticleYear
Real-Time PCR for Gene Expression Quantification in Asthma.
    Methods in molecular biology (Clifton, N.J.), 2016, Volume: 1434

    The quantitative real-time PCR (qPCR) has become the reference technique for studying gene expression in recent years. The application of qPCR to the study of asthma provides very useful information regarding the gene expression mechanisms. The quantification of RNA from cDNA can be performed by using fluorescent dyes or specific sequence probes. Here, we describe the protocol to quantify gene expression levels using SYBR Green as fluorescent dye. The protocol starts with the RNA extraction, followed by reverse transcription to obtain cDNA, quantification and finally data analysis.

    Topics: Asthma; Benzothiazoles; Diamines; Fluorescent Dyes; Gene Expression Profiling; Gene Expression Regulation; Genetic Predisposition to Disease; Humans; Organic Chemicals; Quinolines; Real-Time Polymerase Chain Reaction; RNA, Messenger

2016