sybr-green-i and Adenovirus-Infections--Human

sybr-green-i has been researched along with Adenovirus-Infections--Human* in 2 studies

Other Studies

2 other study(ies) available for sybr-green-i and Adenovirus-Infections--Human

Article	Year
Detection of adenovirus DNA in throat swabs and blood by SYBR green real-time PCR assay in patients with adenovirus-associated tonsillitis. Japanese journal of infectious diseases, 2006, Volume: 59, Issue:6 The adenovirus DNA load ranged from 10(5) to 10(10) copy/mL and from 10(4) to 10(7) copy/mL in throat swabs and blood from patients with adenovirus-associated exudative tonsillitis, respectively. The copy number of adenovirus DNA in blood was positively correlated with the duration of the fever. Topics: Adenovirus Infections, Human; Adenoviruses, Human; Benzothiazoles; Child; Child, Preschool; Diamines; DNA, Viral; Female; Fluorescent Dyes; Humans; Infant; Male; Organic Chemicals; Pharynx; Polymerase Chain Reaction; Quinolines; Specimen Handling; Tonsillitis	2006
Detection of adenovirus DNA in clinical samples by SYBR Green real-time polymerase chain reaction assay. Pediatrics international : official journal of the Japan Pediatric Society, 2005, Volume: 47, Issue:3 Adenoviruses are associated with a variety of diseases including upper respiratory tract infections, acute conjunctivitis, cystitis and gastroenteritis. Adenoviruses can also cause fatal disseminated infections in patients undergoing stem cell transplantation. Measurement of adenovirus load in clinical samples from localized adenovirus infections or disseminated adenovirus infections may provide important information for analyzing the pathogenesis of various adenovirus infections. The purpose of the present study was to develop and optimize a highly sensitive real-time polymerase chain reaction (PCR) assay to detect a wide range of adenoviruses and to detect adenovirus DNA in clinical samples from immunocompetent children.. Clinical samples of throat swabs and blood were collected from 111 patients suspected of having adenovirus infection. The copy number of adenovirus DNA was measured by real-time PCR assay.. SYBR Green real-time PCR assay is able to detect 10-10(6) copies of standard adenovirus DNA per run. Adenovirus DNA was detected in all culture-positive samples serotyped as 1, 2, 3, 4, 5, 6, 8 and 11. Viral loads on throat swabs from immunocompetent children with adenovirus infection ranged from 10(5) to 10(11) copies/mL. Adenovirus DNA was detected in 60% of blood samples and copy number ranged from 10(3) to 10(5) copies/mL.. SYBR Green real-time PCR is a useful quantitative tool for analysis of adenovirus DNA. The present results for immunocompetent children with adenovirus infections provided basic data for comparison with data obtained from immunocompromised patients. Topics: Adenovirus Infections, Human; Adenoviruses, Human; Benzothiazoles; Child, Preschool; Diamines; DNA, Viral; Female; Fluorescent Dyes; Humans; Male; Organic Chemicals; Pharynx; Polymerase Chain Reaction; Quinolines	2005

Article

Year

Detection of adenovirus DNA in throat swabs and blood by SYBR green real-time PCR assay in patients with adenovirus-associated tonsillitis.

Japanese journal of infectious diseases, 2006, Volume: 59, Issue:6

The adenovirus DNA load ranged from 10(5) to 10(10) copy/mL and from 10(4) to 10(7) copy/mL in throat swabs and blood from patients with adenovirus-associated exudative tonsillitis, respectively. The copy number of adenovirus DNA in blood was positively correlated with the duration of the fever.

Topics: Adenovirus Infections, Human; Adenoviruses, Human; Benzothiazoles; Child; Child, Preschool; Diamines; DNA, Viral; Female; Fluorescent Dyes; Humans; Infant; Male; Organic Chemicals; Pharynx; Polymerase Chain Reaction; Quinolines; Specimen Handling; Tonsillitis

2006

Detection of adenovirus DNA in clinical samples by SYBR Green real-time polymerase chain reaction assay.

Pediatrics international : official journal of the Japan Pediatric Society, 2005, Volume: 47, Issue:3

Adenoviruses are associated with a variety of diseases including upper respiratory tract infections, acute conjunctivitis, cystitis and gastroenteritis. Adenoviruses can also cause fatal disseminated infections in patients undergoing stem cell transplantation. Measurement of adenovirus load in clinical samples from localized adenovirus infections or disseminated adenovirus infections may provide important information for analyzing the pathogenesis of various adenovirus infections. The purpose of the present study was to develop and optimize a highly sensitive real-time polymerase chain reaction (PCR) assay to detect a wide range of adenoviruses and to detect adenovirus DNA in clinical samples from immunocompetent children.. Clinical samples of throat swabs and blood were collected from 111 patients suspected of having adenovirus infection. The copy number of adenovirus DNA was measured by real-time PCR assay.. SYBR Green real-time PCR assay is able to detect 10-10(6) copies of standard adenovirus DNA per run. Adenovirus DNA was detected in all culture-positive samples serotyped as 1, 2, 3, 4, 5, 6, 8 and 11. Viral loads on throat swabs from immunocompetent children with adenovirus infection ranged from 10(5) to 10(11) copies/mL. Adenovirus DNA was detected in 60% of blood samples and copy number ranged from 10(3) to 10(5) copies/mL.. SYBR Green real-time PCR is a useful quantitative tool for analysis of adenovirus DNA. The present results for immunocompetent children with adenovirus infections provided basic data for comparison with data obtained from immunocompromised patients.

Topics: Adenovirus Infections, Human; Adenoviruses, Human; Benzothiazoles; Child, Preschool; Diamines; DNA, Viral; Female; Fluorescent Dyes; Humans; Male; Organic Chemicals; Pharynx; Polymerase Chain Reaction; Quinolines

2005