sulfated-pentagalloylglucoside and Keratitis--Herpetic

Herpes simplex virus type-1 (HSV-1) is a significant pathogen that affects vision by targeting multiple regions in the human eye including iris. Using a focused library of synthetic non-saccharide glycosaminoglycan mimetics (NSGMs), we identified sulfated pentagalloylglucoside (SPGG) as a potent inhibitor of HSV-1 entry and cell-to-cell spread in the primary cultures of human iris stromal (HIS) cells isolated from eye donors. Using in vitro β-galactosidase reporter assay and plaque reduction assay, SPGG was found to inhibit HSV-1 entry in a dosage-dependent manner (IC

Topics: Cells, Cultured; Glucosides; Glycosaminoglycans; Herpesvirus 1, Human; High-Throughput Screening Assays; Humans; Inhibitory Concentration 50; Iris; Keratitis, Herpetic; Small Molecule Libraries; Stromal Cells; Structure-Activity Relationship; Sulfuric Acid Esters; Virus Internalization