succinoglycan and Sarcoma-180

Natural polysaccharides have attracted considerable interests due to diverse biological activities. Succinoglycan is an extracellular polysaccharide produced by most Agrobacterium strains. Here, we confirmed riclin was a typical succinoglycan by NMR and methylation analysis, and investigated the antitumor effects of riclin in sarcoma 180 tumor-bearing mice. The results showed that riclin inhibited the tumor growth significantly as well as cyclophosphamide (CTX). While CTX caused serious damage to spleen structure, riclin increased the spleen index and promoted lymphocytes proliferation in peripheral blood, spleen and lymph nodes. Riclin decreased splenocytes apoptosis as evidenced by alterations of B-cell lymphoma-2 family proteins and Cleaved Caspase-3 protein. Moreover,

Topics: Agrobacterium; Animals; Antineoplastic Agents; Apoptosis; Carbohydrate Sequence; Caspase 3; Cell Proliferation; Cyclophosphamide; Gene Expression Regulation, Neoplastic; Immunologic Factors; Lymph Nodes; Lymphocytes; Male; Metabolome; Methylation; Mice; Mice, Inbred C57BL; Polysaccharides, Bacterial; Proto-Oncogene Proteins c-bcl-2; Sarcoma 180; Spleen; Tumor Burden