strontium-radioisotopes and Foreign-Bodies

Under the influence of ionizing radiation on hematopoietic system, the level of its injury is determined not only by the radiosensitivity of hematopoietic stem cells, but also by radiation induced changes in microenvironment func tioning, in particular, mesenchymal stem cells as its components.. to define functioning characteristics of mesenchymal stem and progenitor cells of rats' bone marrow under prolonged action of ionizing radiation as a result of 90Sr incorporation.. We applied the model of Wistar rats' internal irradiation with 90Sr radionuclide and per formed the in vitro cultivation of their bone marrow mesenchymal cells. Colony forming efficiency in the in vitro cell culture was determined, as well as the possibility of these cells to form feeder layers and to support rat bone mar row hematopoietic cells in the culture of diffusion chambers in vitro.. We established that chronic action of incorporated 90Sr radionuclide induced considerable decrease in proliferative activity of mesenchymal stem cells comparing to control, as well as the inhibition of the capability to prolonged support of hematopoietic processes in vitro by their feeder layers.Thus, bone marrow mesenchymal stem cells and their closest progeny - progenitor cells were characterized by rather high radiosensitivity under the influence of ionizing radiation, which was revealed in considerable decline of their functional activity in cell culture in vitro comparing to control indices as a result of irradiation.. Pry diï ionizuiuchogo vyprominiuvannia na krovotvornu systemu stupin' ïï urazhennia vyznachaiet'sia ne lyshe radio chutlyvistiu gemopoetychnykh stovburovykh klityn, a y̆ radiatsiy̆no indukovanymy zminamy u funktsionuvanni mikro otochennia, zokrema, mezenkhimal'nykh stovburovykh klityn iak y̆ogo skladovykh.Meta roboty: vyznachyty osoblyvosti funktsionuvannia mezenkhimal'nykh stovburovykh klityn ta klityn popered nykiv kistkovogo mozku shchuriv v umovakh dovgotryvaloï diï ionizuiuchoï radiatsiï vnaslidok inkorporatsiï 90Sr. Materialy i metody. Bulo vykorystano model' vnutrishn'ogo oprominennia radionuklidom 90Sr shchuriv Vistar ta zdiy̆sneno kul'tyvuvannia in vitro mezenkhimal'nykh klityn ïkhn'ogo kistkovogo mozku. Vyznachaly pokaznyky efektyv nosti koloniieutvorennia u kul'turi in vitro ta zdatnist' tsykh klityn formuvaty fidernyy̆ shar i pidtrymuvaty kro votvorni klityny kistkovogo mozku shchuriv u kul'turi dyfuziy̆nykh kamer in vitro.Rezul'taty ta vysnovky. Z’iasovano, shcho khronichna diia inkorporovanogo radionuklidu 90Sr zumovliuvala suttieve zmenshennia u porivnianni z kontrolem proliferatyvnoï aktyvnosti mezenkhimal'nykh stovburovykh klityn, a takozh prygnichennia zdatnosti sformovanykh nymy fidernykh shariv do dovgotryvaloï pidtrymky protsesiv gemopoezu in vitro.Otzhe, mezenkhimal'ni stovburovi klityny kistkovogo mozku ta ïkhni nay̆blyzhchi nashchadky – klityny poperednyky pry diï ionizuiuchoï radiatsiï kharakteryzuvalys' dovoli vysokoiu radiochutlyvistiu, shcho proiavlialos' u suttievomu znyzhenni vnaslidok oprominennia ïkhn'oï funktsional'noï aktyvnosti u kul'turi in vitro porivniano z kontrolem.

Topics: Animals; Bone Marrow; Cell Proliferation; Colony-Forming Units Assay; Diffusion Chambers, Culture; Feeder Cells; Foreign Bodies; Hematopoietic Stem Cells; Mesenchymal Stem Cells; Primary Cell Culture; Radiation Injuries; Radiation Tolerance; Radiation, Ionizing; Rats; Rats, Wistar; Strontium Radioisotopes

Topics: Animals; Electron Probe Microanalysis; Female; Foreign Bodies; Foreign-Body Migration; Foreign-Body Reaction; Macaca; Male; Polytetrafluoroethylene; Strontium Radioisotopes; Urethra