strontium-radioisotopes and Cell-Transformation--Neoplastic

Topics: Animals; B-Lymphocytes; Cell Transformation, Neoplastic; Erythropoiesis; Friend murine leukemia virus; Genes; Immune Tolerance; Immunity, Cellular; Immunity, Innate; Immunosuppression Therapy; Killer Cells, Natural; Leukemia, Erythroblastic, Acute; Lymphocyte Activation; Macrophages; Mice; Strontium Radioisotopes; T-Lymphocytes; T-Lymphocytes, Regulatory

Rats were treated with various doses of 89Strontium. At a dose effecting depletion of the bone marrow (200 muCi), NK activity of spleen, peritoneal and peripheral blood lymphocytes was unaltered or only slightly decreased. At higher doses (400 muCi), a general impairment of the immune system, including T-cell functions, was observed. After application of 700 muCi 89Strontium, rats died within three weeks.

Topics: Animals; Bone Marrow; Cell Transformation, Neoplastic; Cytotoxicity, Immunologic; Dose-Response Relationship, Immunologic; Killer Cells, Natural; Lymphocyte Activation; Neoplasms, Experimental; Radiation Chimera; Rats; Rats, Inbred Strains; Strontium Radioisotopes; T-Lymphocytes

Cloned cell lines were established from two swine with radiation-induced myeloproliferative disorders, including one cell culture from an animal with myelogenous leukemia and one from an animal with myeloid metaplasia. A third cloned cell line with similar morphology was established from pooled normal fetal swine cornea to compare the growth characteristics of cells from normal and irradiated swine. All three cell lines grew as foci of aggregated cells and were able to form macroscopic colonies in semisolid agar medium. The lack of normal mechanisms of contact inhibition and the observed aneuploidy indicated that these cells were morphologically transformed. Further, the cloned cells caused tumors in nude mice, clearly indicating that these cells were also malignantly transformed. A major difference between these cell lines was that type C viruses were observed only in the cells derived from swine with myeloproliferative disorders.

Topics: Animals; Cell Division; Cell Line; Cell Transformation, Neoplastic; Clone Cells; Cornea; Karyotyping; Leukemia, Myeloid; Leukemia, Radiation-Induced; Mice; Neoplasms, Experimental; Primary Myelofibrosis; Radiation Injuries, Experimental; Strontium Radioisotopes; Swine

Topics: Animals; Antigens, Viral; Avian Leukosis Virus; Avian Sarcoma Viruses; Cell Line; Cell Transformation, Neoplastic; Cells, Cultured; Chickens; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Epitopes; Female; Fibroblasts; Immune Sera; Immunodiffusion; Iodine Radioisotopes; Isoelectric Focusing; Male; Rabbits; Radioimmunoassay; Retroviridae; Strontium Radioisotopes; Viral Proteins

Topics: Adenoviridae; Animals; Cell Line; Cell Nucleus; Cell Transformation, Neoplastic; Culture Techniques; Cytopathogenic Effect, Viral; DNA Viruses; Inclusion Bodies, Viral; Kidney; Leukemia, Myeloid; Leukemia, Radiation-Induced; Microscopy, Electron; Retroviridae; Strontium Radioisotopes; Swine; Swine Diseases