streptothricins and Cell-Transformation--Neoplastic

Two streptothricin-group antibiotics, AN-201 I and II, were newly discovered and isolated from the culture broth of Streptomyces nojiriensis C-13. These antibiotics were purified by IRC-50 (H+) and CM-Sephadex C-50 chromatography, and paper electrophoresis. Structural analysis of AN-201 I and II showed that they were N beta-acetylated derivatives of streptothricin E and D, respectively. They had antibacterial activities against several strains of Escherichia coli, Bacillus subtilis, Micrococcus luteus and Staphylococcus aureus, and showed a strong selective cytotoxic effect on 3T3 cells transformed with SV-40 as compared with their normal cells in a test system in vitro as well as in vivo.

Topics: Animals; Anti-Bacterial Agents; Antibiotics, Antineoplastic; Cell Line; Cell Transformation, Neoplastic; Cells, Cultured; Drug Evaluation, Preclinical; Fermentation; Lung Neoplasms; Mice; Microbial Sensitivity Tests; Simian virus 40; Streptomyces; Streptothricins; Structure-Activity Relationship