stilbenes and Tendinopathy

stilbenes has been researched along with Tendinopathy* in 1 studies

Other Studies

1 other study(ies) available for stilbenes and Tendinopathy

ArticleYear
Sirt-1 is required for the inhibition of apoptosis and inflammatory responses in human tenocytes.
    The Journal of biological chemistry, 2012, Jul-27, Volume: 287, Issue:31

    Tendon overuse injuries and tendinitis are accompanied by catabolic processes and apoptosis of tenocytes. However, the precise molecular mechanisms of the destructive processes in tendon are not fully understood. Sirt-1, a nicotinamide adenine dinucleotide (NAD(+))-dependent deacetylase, has been linked to transcriptional silencing and appears to play a key role in inflammation. The purpose of this study was to examine whether down-regulation of Sirt-1 using antisense oligonucleotides (ASO) affects inflammatory and apoptotic signaling in tenocytes. Transient transfection of tenocytes with ASO against Sirt-1 induced expression of Bax and other proteins involved in apoptosis (cleaved caspase-3 and poly(ADP-ribose)polymerase), acetylation of tumor suppressor p53, and mitochondrial degradation. Interestingly, Sirt-1 was found to interact directly with p53. In contrast, Sirt-1 activator resveratrol inhibited interleukin-1β (IL-1β)- and nicotinamide-induced NF-κB activation and p65 acetylation and suppressed the activation of IκB-α kinase. Resveratrol also reversed the IL-1β- or nicotinamide-induced up-regulation of various gene products that mediate inflammation (cyclooxygenase-2) and matrix degradation (matrix metalloproteinase-9) that are known to be regulated by NF-κB. Knockdown of Sirt-1 by using ASO abolished the inhibitory effects of resveratrol on inflammatory and apoptotic signaling including Akt activation and SCAX suppression. Down-regulation of histone deacetylase Sirt-1 by mRNA interference abrogated the effect of resveratrol on NF-κB suppression, thus highlighting the crucial homeostatic role of this enzyme. Overall, these results suggest for the first time that Sirt-1 can regulate p53 and NF-κB signaling via deacetylation, demonstrating a novel role for resveratrol in the treatment of tendinitis/tendinopathy.

    Topics: Acetylation; Apoptosis; bcl-2-Associated X Protein; Caspase 3; Cell Proliferation; Cell Survival; Cells, Cultured; Enzyme Activation; Enzyme Activators; Gene Knockdown Techniques; Humans; Interleukin-1beta; Mitochondria; NF-kappa B; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) Polymerases; Protein Binding; Protein Processing, Post-Translational; Proteolysis; Proto-Oncogene Proteins c-akt; Resveratrol; RNA Interference; RNA, Small Interfering; Signal Transduction; Sirtuin 1; Stilbenes; Tendinopathy; Tendons; Tumor Suppressor Protein p53

2012