stilbenes and Laryngeal-Neoplasms

Polydatin (PD), a stilbene compound extracted from Polygonum cuspidatum, is suggested to possess anti-cancer activities, including inhibition of cell proliferation, cell cycle arrest, and induction of apoptosis. The platelet-derived growth factor (PDGF)/AKT signaling pathway plays complex roles in tumor suppression. However, the effect of PD on the PDGF/AKT signaling pathway in laryngeal cancer and HeLa cells has not been explored. MTT assay and flow cytometry showed that PD inhibited cell proliferation and induced apoptosis in Hep-2 and AMC-HN-8 cells. Western blot analysis indicated that PD inhibited the expression levels of PDGF-B and phosphorylated AKT (p-AKT) in both cells. Treatment of PDGF-B siRNA or PDGFR inhibitor found that after the PDGF signaling was inactivated, p-AKT expression was significantly decreased in Hep-2 cells. Tumor xenograft experiment in nude mice indicated PD significantly inhibited the growth of Hep-2 cells in vivo. In conclusion, PD inhibited cell proliferation and induced apoptosis in laryngeal cancer and HeLa cells via inactivation of the PDGF/AKT signaling pathway.

Topics: Animals; Cell Proliferation; Fallopia japonica; Female; Glucosides; HeLa Cells; Humans; Laryngeal Neoplasms; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Platelet-Derived Growth Factor; Proto-Oncogene Proteins c-akt; Signal Transduction; Stilbenes; Xenograft Model Antitumor Assays

Laryngeal cancer is one of the most common malignant tumors in the respiratory tumors, and its incidence ranks second highest in the respiratory tumors. Resveratrol (Res) is a kind of polyphenols, which can inhibit nucleotides can inhibit the growth of liver cancer cells, gastric cancer cells, pancreatic cells and other tumor cells by inhibiting ribonucleotide reductase in the cells. Taxol (Tax) is a kind of secondary metabolites of Taxus chinensis, which has anti-tumor activity for breast cancer, cervical cancer, ovarian cancer and other tumors by inhibiting cellular microtubule depolymerization. But at present the effects of resveratrol combined with taxol on human laryngeal carcinoma cell strain Hep-2 and their underlying molecular mechanisms are rarely reported. After human laryngeal cancer cell Hep-2 cells were processed with resveratrol (Res) and taxol (Tax), CCK-8 assay was used to evaluate the effect of these two herbs on the proliferation of cancer cells; AO/PI staining and JC-1 were used to detect Hep-1 cells apoptosis; the expression of Bax, Bcl-2, PARP, TRIB3, and XIAP genes was detected by real time quantitative PCR; the activity of caspase-3 and caspase-8 was determined with quantitative fluorescence method. The experimental results showed that compared with Tax, Res medication alone, joint group significantly enhanced inhibition of Hep-2 cells activity, decreased the dosage of Tax, increased the expression of Bax and PARP, TRIB3, reduced the expression of the Bcl-2 and XIAP, and promoted the activity of caspase-3 and caspase-8. The test results showed that compared with the single medication, combined group could significantly increase the inhibitory effect on Hep-2 cells, significantly reduce Tax dosage, increase expressions of Bax, PARP, TRIB3, reduce expressions of Bcl-2, XIAP, and promote activity of caspase-3, caspase-8. This indicated apoptosis of human laryngeal carcinoma cell strain Hep-2 may be induced with Res, Tax, and the combination of these two herbs by mitochondria pathway. It provides valuable clue for further research on combination of Res and Tax for the treatment of laryngeal cancer, and expanding the combined application of Res and Tax.

Topics: Antineoplastic Agents; Apoptosis; bcl-2-Associated X Protein; Caspase 8; Cell Line, Tumor; Humans; Laryngeal Neoplasms; Paclitaxel; Proto-Oncogene Proteins c-bcl-2; Resveratrol; Stilbenes

To explore the immunological mechanism of the anti-tumor function of resveratrol (Res) through in vitro or vivo experiments.. The tumor cell growth repression rate was measured by MTT colorimetry. Flow cytometry was used to analyze the cell cycle of Hep2 cells (laryngeal squamous cancer cell line). The immune function and cytokine levels in the serum of the tumor-bearing mice were determined by MTT colorimetry, hemolysis spectrophotography, improved Mayer's method and ELISA.. Res inhibited the tumor cell growth and enhanced the apoptosis of tumor cells in time-concentration-dependent manners showing the phenomenon of obvious G(0)/G(1) blocking and apoptotic peak. The maximal tumor inhibition rate came up to 42.76%. Furthermore, Res improved function of T, B lymphocytes, killing activity of NK cells, release of antibodies, and the total complement activity in serum. It also increased contents of IL-2 and TGF-beta1 but reduced that of IL-8 and VEGF.. Res can not only affect tumor cells directly but also exert anti-tumor efficiency through reinforcing cell-mediated, humoral immune response and accommodating lymphocytes to secrete cytokines.

Topics: Animals; Antibody Formation; Antineoplastic Agents; Apoptosis; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Flow Cytometry; Humans; Interleukin-2; Interleukin-8; Laryngeal Neoplasms; Mice; Resveratrol; Stilbenes; Transforming Growth Factor beta1; Vascular Endothelial Growth Factor A

To find a new kind of biological response modifier to anti-laryngocarcinoma through studying the efficiency of resveratrol.. To detect the growth inhibiting rate of Hep-2 exposed to resveratrol with MTT colorimetry and to analyze the apoptosis and cell cycle of Hep-2 with flow cytometry. As having formed the model of laryngocarcinoma, quite a few indicatrixes including the content changes of IL-2, IL-8,TGF-beta1 and VEGF were detected.. Resveratrol could induce apoptosis of Hep-2 in time-concentration-dependent manners showing obvious cell cycle blocking of G0/G1 and apoptotic peak. Furthermore, resveratrol improved remarkably the growth condition of mice planted with Hep-2 while enhancing their immunological function.. As a kind of biological response modifier, resveratrol could strengthen anti-tumor immunoresponse, accommodating lymphocyte to secrete cytokine, through which it exerted the efficiency of anti-laryngocarcinoma.

Topics: Animals; Antineoplastic Agents, Phytogenic; Apoptosis; Cell Cycle; Cell Line, Tumor; Humans; Interleukin-2; Interleukin-8; Laryngeal Neoplasms; Male; Mice; Mice, Inbred C57BL; Resveratrol; Stilbenes; Transforming Growth Factor beta1; Vascular Endothelial Growth Factor A