stilbenes and Intervertebral-Disc-Displacement

To investigate the regulatory effect of resveratrol (RES) on the extracellular matrix (ECM) expression of nucleus pulposus cells (NPC), and its relative molecular mechanism.. Ten patients receiving discectomy were collected, of which 5 patients were young with spinal burst fracture, classified as control group; the rest 5 patients were senile with lumbar disc herniation, classified as degenerative group. The nucleus pulposus tissue of 2 groups were collected, the. Immunohistochemical staining and Western blot detection showed that when compared with control group, the cell proportion of expression of β-catenin were significantly increased in degenerative group (. RES regulates the ECM expression of NPC via Wnt/β-catenin signaling pathway, which provide a new idea for intervertebral disc degeneration disease treatment.. 探讨白藜芦醇（resveratrol，RES）对退变髓核细胞（nucleus pulposus cells，NPC）细胞外基质（extracellular matrix，ECM）表达的调控作用及其相关分子机制。.. 选取临床上接受椎间盘摘除术的 10 例患者，其中 5 例为年轻脊柱爆裂骨折患者，作为对照组；余 5 例为老年腰椎间盘突出症患者，作为退变组。取两组患者髓核组织，免疫组织化学染色比较 β-catenin 的原位表达，Western blot 检测 β-catenin、Ⅱ型胶原和聚集蛋白聚糖（Aggrecan）蛋白表达。取退变组髓核组织分离、培养 NPC，分别用 IL-1β 单独（B 组）或联合 RES（C 组）刺激第 3 代 NPC，并设未刺激细胞作为空白对照组（A 组），Western blot 检测Ⅱ型胶原和 Aggrecan 蛋白表达。进一步采用小干扰 RNA（small interfering RNA，siRNA）靶向沉默 SIRT1 和 β-catenin 后，采用 Western blot、实时荧光定量 PCR 检测 β-catenin、SIRT1 蛋白和基因表达。用完全培养基（1 组）、IL-1β（2 组）、RES+IL-1β（3 组）、SIRT1-siRNA+RES+IL-1β（4 组）刺激第 3 代 NPC 培养 24 h 后，细胞免疫荧光染色检测 β-catenin 核转位情况；用完全培养基（Ⅰ组）、IL-1β（Ⅱ组）、IL-1β+β-catenin-siRNA（Ⅲ组）、IL-1β+RES（Ⅳ组）、IL-1β+RES+SIRT1-siRNA（Ⅴ组）刺激第 3 代 NPC 培养 24 h 后，采用 Western blot 检测Ⅱ型胶原和 Aggrecan 蛋白表达。.. 免疫组织化学染色及 Western blot 检测示，与对照组比较，退变组髓核组织中 β-catenin 阳性表达的细胞比例显著升高（. RES 可以通过抑制 Wnt/β-catenin 信号通路维持 NPC ECM 的表达，为椎间盘退行性疾病的治疗提供了新思路。.

Topics: Aggrecans; beta Catenin; Cell Count; Cells, Cultured; Collagen Type II; Diskectomy; Extracellular Matrix; Gene Expression; Humans; Interleukin-1beta; Intervertebral Disc Degeneration; Intervertebral Disc Displacement; Nucleus Pulposus; Resveratrol; Sirtuin 1; Stilbenes