stilbenes and Gallbladder-Neoplasms

Gallbladder cancer is the most common malignant tumor of the biliary system and is characterized by difficulty to diagnose in early stages, a high degree of malignancy, and poor prognosis. Finding new drugs may improve the prognosis for this dismal cancer. Herein, we investigated the potential application of pterostilbene (PTS) against gallbladder cancer in vivo and in vitro. PTS potently inhibited cell proliferation, migration and invasion of gallbladder cancer cells. Moreover, PTS also had a function of inducing apoptosis in vitro. Meanwhile, PTS reversed EMT with a correlated inhibition of PI3K/Akt activation. Tumor xenograft models showed that PTS inhibited tumor growth and had low toxicity in vivo, which were consistent with the in vitro data. These findings indicate that PTS arrests cell growth through inhibition of PI3K/AKT signaling and is a potential drug for the therapy of gallbladder cancer.

Topics: Animals; Antineoplastic Agents, Phytogenic; Apoptosis; Cell Line, Tumor; Cell Movement; Cell Proliferation; Gallbladder Neoplasms; Humans; Male; Mice; Mice, Nude; Phosphatidylinositol 3-Kinases; Proto-Oncogene Proteins c-akt; Signal Transduction; Stilbenes

To explore the contribution of resveratrol (Res) to the proliferation and apoptosis of gallbladder carcinoma (GBC) cell line as well as fibroblast 3T3 cell line in vitro.. The tumor cell growth repressing rate was measured by the MTT method,and flow cytometry was used to analyse cell cycle. The gene expression of bcl-2 c-myc p53 were examined by SABC.. Res obviously suppressed the proliferation (P <0. 01) in concentration and time dependent manner, it's tiptop was 54%. Res could induce apoptosis of tumor cells. The maximum apoptosis rate of tumor was 30. 52%, experiment group compared with comparison group Gl's cells rised from 34. 88% to 55.47 +/- 3. 95%, S's cells reduced 8.41 - 17.54%, which showed obvious phenomenon of G0/G1 blocking. The GBC cell's bcl-2, c-myc gene reduced expression,while p53 gene increased expression.. The results of this study confirm the ability of Res to suppress the proliferation of gallbladder carcinoma (GBC) cell with a typical apoptotic feature in vitro. Therefore, Res may be considered as a possible treatment strategy for gallbladder carcinoma.

Topics: Animals; Antineoplastic Agents, Phytogenic; Apoptosis; Cell Cycle; Cell Line; Cell Line, Tumor; Cell Proliferation; Dose-Response Relationship, Drug; Fibroblasts; Flow Cytometry; Gallbladder Neoplasms; Humans; Immunohistochemistry; Mice; Plants, Medicinal; Resveratrol; Stilbenes