sq-23377 and Intestinal-Diseases--Parasitic

The cellular immune response was evaluated in a C3H/HeJ mouse model of intestinal amebiasis at 5-60 days postinoculation with Entamoeba histolytica. At various intervals, spleen lymphocytes were obtained from infected and noninfected control mice and cultured with concanavalin A (Con A), pokeweed mitogen (PWM), or ameba antigen. E. histolytica infection induced a cyclic depression of DNA synthesis when spleen lymphocytes were stimulated with a T-cell mitogen (Con A), a T- and B-cell (PWM) mitogen, or an ameba antigen. A similar response was observed in the determinations of interleukin-2 in the supernatants of Con A-stimulated spleen cells from infected mice. When spleen cells from E. histolytica-infected mice were stimulated with phorbol myristate acetate plus ionomycin, results indicated a signal-transduction defect. These alterations, observed at the cellular level, might facilitate invasion of the host by the parasite.

Topics: Animals; Antigens, Protozoan; Disease Models, Animal; Entamoeba histolytica; Entamoebiasis; Immunity, Cellular; Interleukin-2; Intestinal Diseases, Parasitic; Ionomycin; Lymphocyte Activation; Mice; Mice, Inbred C3H; Mitogens; Signal Transduction; Spleen; Tetradecanoylphorbol Acetate