sq-23377 has been researched along with Disease* in 1 studies
1 other study(ies) available for sq-23377 and Disease
| Article | Year |
|---|---|
In Vitro and In Vivo Pipeline for Validation of Disease-Modifying Effects of Systems Biology-Derived Network Treatments for Traumatic Brain Injury-Lessons Learned.
We developed a pipeline for the discovery of transcriptomics-derived disease-modifying therapies and used it to validate treatments in vitro and in vivo that could be repurposed for TBI treatment. Desmethylclomipramine, ionomycin, sirolimus and trimipramine, identified by in silico LINCS analysis as candidate treatments modulating the TBI-induced transcriptomics networks, were tested in neuron-BV2 microglial co-cultures, using tumour necrosis factor α as a monitoring biomarker for neuroinflammation, nitrite for nitric oxide-mediated neurotoxicity and microtubule associated protein 2-based immunostaining for neuronal survival. Based on (a) therapeutic time window in silico, (b) blood-brain barrier penetration and water solubility, (c) anti-inflammatory and neuroprotective effects in vitro ( Topics: Animals; Anti-Inflammatory Agents; Biomarkers; Brain Injuries, Traumatic; Cell Line; Clomipramine; Coculture Techniques; Cytokines; Disease; Gene Expression; In Vitro Techniques; Ionomycin; Machine Learning; Male; Microglia; Neurons; Neuroprotection; Neuroprotective Agents; NF-E2-Related Factor 2; Nitrites; Rats; Sirolimus; Systems Biology; Transcriptome; Trimipramine; Tumor Necrosis Factor-alpha; Up-Regulation | 2019 |