sphingosine-kinase and Neutropenia

sphingosine-kinase has been researched along with Neutropenia* in 1 studies

Other Studies

1 other study(ies) available for sphingosine-kinase and Neutropenia

ArticleYear
A critical role for sphingosine kinase in anaphylatoxin-induced neutropenia, peritonitis, and cytokine production in vivo.
    Journal of immunology (Baltimore, Md. : 1950), 2005, May-15, Volume: 174, Issue:10

    The aim of our study was to investigate the roles played by sphingosine kinase (SPHK) in the anaphylatoxin C5a-triggered responses in vivo. Our data show that i.v. administration of C5a triggers a rapid neutropenic response, but pretreating mice with the SPHK inhibitor, N,N-dimethylsphingosine (DMS), 10 min before the C5a i.v. administration substantially inhibited the C5a-triggered neutropenia. Similarly the i.v. administration of C5a caused a rapid increase in the serum levels of TNF-alpha and IL-6, and this increase in cytokine levels was blocked by DMS. We then induced acute peritonitis with C5a. The C5a i.p. injection triggered a fast recruitment of neutrophils, later followed by monocytes, into the peritoneal cavity. Vascular permeability was also observed: when we i.v. injected Evans blue before C5a i.p. injection, we could observe a continued influx of the dye into the peritoneum. In mice pretreated with DMS, there was a significant reduction on the C5a-triggered neutrophil and monocyte infiltration, as well as a marked reduction on the Evans blue influx. Our data also show that the i.p. administration of C5a caused a rapid increase in TNF-alpha and IL-6 levels in the peritoneal cavity, and this increase in cytokine levels was substantially inhibited in mice pretreated with the SPHK inhibitor. Taken together, these observations suggest a potential role for SPHK in the C5a-triggered inflammatory responses in vivo.

    Topics: Animals; Capillary Permeability; Chemotaxis, Leukocyte; Complement C5a; Cytokines; Humans; Injections, Intraperitoneal; Interleukin-6; Male; Mice; Mice, Inbred BALB C; Monocytes; Neutropenia; Neutrophil Infiltration; Peritoneal Cavity; Peritoneal Lavage; Peritonitis; Phosphotransferases (Alcohol Group Acceptor); Tumor Necrosis Factor-alpha

2005