sphingosine-1-phosphate and Ovarian-Cysts

We aimed to analyze if anti-apoptotic agent sphingosine-1-phosphate offers protection against in vitro follicle atresia during culture of human ovarian cortical samples.. A translational research study of ex-vivo and in-vitro models of human ovarian tissue.. Ovarian cortical tissue fragments (1 × 0.5 cm) were obtained from young patients (n = 15 mean age ± SD: 29.4 ± 2.5) undergoing laparoscopic excision of benign ovarian cysts. The samples were cultured for 4 days in 24-well format culture plate using conventional culture techniques. S1P was added to culture media at 200 and 400 μM concentrations. At the end of culture period the samples were processed for both histomorphological assessment and detection of apoptosis with immunohistochemistry and western blot methods using apoptosis marker cleaved caspase-3. In vitro estradiol (E. These results suggest that S1P promotes follicle survival in human ovarian cortical samples in vitro.

Topics: Adult; Anti-Mullerian Hormone; Apoptosis; Biomarkers; Blotting, Western; Caspase 3; Cell Survival; Down-Regulation; Enzyme-Linked Immunosorbent Assay; Estradiol; Female; Follicular Atresia; Humans; Immunohistochemistry; Lysophospholipids; Ovarian Cysts; Ovarian Follicle; Protective Agents; Proteolysis; Sphingosine; Tissue Culture Techniques