sphingosine-1-phosphate and Keratoconus

sphingosine-1-phosphate has been researched along with Keratoconus* in 1 studies

Other Studies

1 other study(ies) available for sphingosine-1-phosphate and Keratoconus

Article	Year
Analysis of sphingolipids in human corneal fibroblasts from normal and keratoconus patients. Journal of lipid research, 2017, Volume: 58, Issue:4 The pathophysiology of human keratoconus (KC), a bilateral progressive corneal disease leading to protrusion of the cornea, stromal thinning, and scarring, is not well-understood. In this study, we investigated a novel sphingolipid (SPL) signaling pathway through which KC may be regulated. Using human corneal fibroblasts (HCFs) and human KC cells (HKCs), we examined the SPL pathway modulation. Both cell types were stimulated by the three transforming growth factor (TGF)-β isoforms: TGF-β1 (T1), TGF-β2 (T2), and TGF-β3 (T3). All samples were analyzed using lipidomics and real-time PCR. Our data showed that HKCs have increased levels of signaling SPLs, ceramide (Cer), and sphingosine 1-phosphate (S1P). Treatment with T1 reversed the increase in Cer in HKCs and treatment with T3 reversed the increase in S1P. Topics: Cell Line; Ceramides; Cornea; Fibroblasts; Fingolimod Hydrochloride; Humans; Keratoconus; Lysophospholipids; RNA, Messenger; Signal Transduction; Sphingolipids; Sphingosine; Transforming Growth Factor beta1; Transforming Growth Factor beta2; Transforming Growth Factor beta3	2017

Article

Year

Analysis of sphingolipids in human corneal fibroblasts from normal and keratoconus patients.

Journal of lipid research, 2017, Volume: 58, Issue:4

The pathophysiology of human keratoconus (KC), a bilateral progressive corneal disease leading to protrusion of the cornea, stromal thinning, and scarring, is not well-understood. In this study, we investigated a novel sphingolipid (SPL) signaling pathway through which KC may be regulated. Using human corneal fibroblasts (HCFs) and human KC cells (HKCs), we examined the SPL pathway modulation. Both cell types were stimulated by the three transforming growth factor (TGF)-β isoforms: TGF-β1 (T1), TGF-β2 (T2), and TGF-β3 (T3). All samples were analyzed using lipidomics and real-time PCR. Our data showed that HKCs have increased levels of signaling SPLs, ceramide (Cer), and sphingosine 1-phosphate (S1P). Treatment with T1 reversed the increase in Cer in HKCs and treatment with T3 reversed the increase in S1P.

Topics: Cell Line; Ceramides; Cornea; Fibroblasts; Fingolimod Hydrochloride; Humans; Keratoconus; Lysophospholipids; RNA, Messenger; Signal Transduction; Sphingolipids; Sphingosine; Transforming Growth Factor beta1; Transforming Growth Factor beta2; Transforming Growth Factor beta3

2017