sphingosine-1-phosphate and Burns

sphingosine-1-phosphate has been researched along with Burns* in 1 studies

Other Studies

1 other study(ies) available for sphingosine-1-phosphate and Burns

Article	Year
Effect of sphingosine 1-phosphate on morphological and functional responses in endothelia and venules after scalding injury. Burns : journal of the International Society for Burn Injuries, 2009, Volume: 35, Issue:8 The uncontrolled increase of vascular permeability is the major obstacle in treatment of severe burns. Sphingosine 1-phosphate (S1P) has emerged as an important modulator of EC barrier function. This study was designed to explore the effect of S1P on morphological alteration in cultured endothelial cells (ECs) after burned plasma stimulation, and second to investigate the hyper-permeability response in intact vessels after scalding injury.. The distribution of VE-cadherin and F-actin was observed by double staining in primary cultured human umbilical vein endothelial cells (HUVECs) with immunofluorescence and fluorescent probes; respectively. Permeability changes were measured by a fluorescence ratio technique in isolated venules from rat skin. Burned plasma was obtained from a third-degree scald covering 30% of the total body surface area.. The intervention with burned plasma on injured rats cultured HUVECs caused a significant disruption of intercellular adherens junction labeled by VE-cadherin staining, accompanied by the formation of F-actin stress fibers in the cells. S1P prevented or reversed these burned plasma-induced morphological alterations in cultured endothelial cells. The inhibition of S1P synthesis with N,N-dimethylsphingosine (DMS) mimicked the burned plasma-evoked redistribution of VE-cadherin and reorganization of F-actin. Venules isolated from burned rats demonstrated similar endothelial cytoskeleton changes with cultured cells under the influence of S1P or DMS. Both pre- and post-burn application of S1P attenuated increased permeability in isolated and perfused skin venules after burned plasma stimulation.. Our results indicate that S1P plays a role in maintaining basal vascular barrier function and could be protective in burn injury by enhancing the endothelial barrier function. Topics: Actins; Adherens Junctions; Animals; Antigens, CD; Burns; Cadherins; Capillary Permeability; Cells, Cultured; Cytoskeleton; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Endothelium, Vascular; Humans; Lysophospholipids; Plasma; Rats; Rats, Wistar; Sphingosine; Venules	2009

Article

Year

Effect of sphingosine 1-phosphate on morphological and functional responses in endothelia and venules after scalding injury.

Burns : journal of the International Society for Burn Injuries, 2009, Volume: 35, Issue:8

The uncontrolled increase of vascular permeability is the major obstacle in treatment of severe burns. Sphingosine 1-phosphate (S1P) has emerged as an important modulator of EC barrier function. This study was designed to explore the effect of S1P on morphological alteration in cultured endothelial cells (ECs) after burned plasma stimulation, and second to investigate the hyper-permeability response in intact vessels after scalding injury.. The distribution of VE-cadherin and F-actin was observed by double staining in primary cultured human umbilical vein endothelial cells (HUVECs) with immunofluorescence and fluorescent probes; respectively. Permeability changes were measured by a fluorescence ratio technique in isolated venules from rat skin. Burned plasma was obtained from a third-degree scald covering 30% of the total body surface area.. The intervention with burned plasma on injured rats cultured HUVECs caused a significant disruption of intercellular adherens junction labeled by VE-cadherin staining, accompanied by the formation of F-actin stress fibers in the cells. S1P prevented or reversed these burned plasma-induced morphological alterations in cultured endothelial cells. The inhibition of S1P synthesis with N,N-dimethylsphingosine (DMS) mimicked the burned plasma-evoked redistribution of VE-cadherin and reorganization of F-actin. Venules isolated from burned rats demonstrated similar endothelial cytoskeleton changes with cultured cells under the influence of S1P or DMS. Both pre- and post-burn application of S1P attenuated increased permeability in isolated and perfused skin venules after burned plasma stimulation.. Our results indicate that S1P plays a role in maintaining basal vascular barrier function and could be protective in burn injury by enhancing the endothelial barrier function.

Topics: Actins; Adherens Junctions; Animals; Antigens, CD; Burns; Cadherins; Capillary Permeability; Cells, Cultured; Cytoskeleton; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Endothelium, Vascular; Humans; Lysophospholipids; Plasma; Rats; Rats, Wistar; Sphingosine; Venules

2009