sorbicillin and Leukemia-P388

Five new nitrogen-containing sorbicillinoids named sorbicillamines A-E (1-5) were isolated from an agitated culture of the deep-sea-derived fungus Penicillium sp. F23-2, which has previously produced indole alkaloids and terpenoids when cultured under static conditions. The structures of 1 to 5, including absolute configurations, were determined based on MS, NMR, and circular dichroism (CD) data. The cytotoxicities of the five new sorbicillin alkaloids against the HeLa, BEL-7402, HEK-293, HCT-116, and P388 cell lines were evaluated.

Topics: Animals; Antineoplastic Agents; Circular Dichroism; Drug Screening Assays, Antitumor; HCT116 Cells; HEK293 Cells; HeLa Cells; Humans; Indole Alkaloids; Leukemia P388; Molecular Structure; Nitrogen; Nuclear Magnetic Resonance, Biomolecular; Oceans and Seas; Penicillium; Resorcinols

Two new acid sorbicillin analogues, (E)-6-(2,4-dihydroxyl-5-methylphenyl)-6-oxo-2-hexenoic acid (1) and 6-(2,4-dihydroxyl-5-methylphenyl)-6-oxohexanoic acid (2), together with 12 known compounds, were isolated from a saline lands-derived fungus Trichoderma sp. The structures of the new compounds were established by interpretation of their spectroscopic data. Their cytotoxic effects on P388 and HL-60 cell lines were preliminarily evaluated by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) method. Furthermore, the new compounds exhibited weak radical scavenging activity against DPPH (1,1-diphenyl-2-picrylhydrazyl).

Topics: Animals; Antineoplastic Agents; Cell Line, Tumor; Free Radical Scavengers; HL-60 Cells; Humans; Leukemia P388; Mice; Resorcinols; Spectrum Analysis; Trichoderma