sodium-pertechnetate-tc-99m and Stomach-Neoplasms

Gastric cancers have poor overall survival despite recent advancements in early detection methods, endoscopic resection techniques, and chemotherapy treatments. Vaccinia viral therapy has had promising therapeutic potential for various cancers and has a great safety profile. We investigated the therapeutic efficacy of a novel genetically-engineered vaccinia virus carrying the human sodium iodide symporter (hNIS) gene, GLV-1 h153, on gastric cancers and its potential utility for imaging with (99m)Tc pertechnetate scintigraphy and ¹²⁴I positron emission tomography (PET).. GLV-1 h153 was tested against five human gastric cancer cell lines using cytotoxicity and standard viral plaque assays. In vivo, subcutaneous flank tumors were generated in nude mice with human gastric cancer cells, MKN-74. Tumors were subsequently injected with either GLV-1 h153 or PBS and followed for tumor growth. (99m)Tc pertechnetate scintigraphy and ¹²⁴I microPET imaging were performed.. GFP expression, a surrogate for viral infectivity, confirmed viral infection by 24 hours. At a multiplicity of infection (MOI) of 1, GLV-1 h153 achieved > 90% cytotoxicity in MNK-74, OCUM-2MD3, and AGS over 9 days, and >70% cytotoxicity in MNK- 45 and TMK-1. In vivo, GLV-1 h153 was effective in treating xenografts (p < 0.001) after 2 weeks of treatment. GLV-1 h153-infected tumors were readily imaged by (99m)Tc pertechnetate scintigraphy and ¹²⁴I microPET imaging 2 days after treatment.. GLV-1 h153 is an effective oncolytic virus expressing the hNIS protein that can efficiently regress gastric tumors and allow deep-tissue imaging. These data encourages its continued investigation in clinical settings.

Topics: Animals; Cell Line, Tumor; Female; Genetic Engineering; Humans; Mice; Mice, Nude; Neoplasm Transplantation; Oncolytic Virotherapy; Oncolytic Viruses; Radionuclide Imaging; Radiopharmaceuticals; Sodium Pertechnetate Tc 99m; Stomach Neoplasms; Symporters; Tumor Burden; Vaccinia virus; Virus Replication

This is a case of striking radioiodine and [99mTc]pertechnetate uptake by disseminated nonthyroidal (gastric) adenocarcinoma. A 65-yr-old man was euthyroid and serum thyroglobulin concentration was normal at 11 ng/ml. Bone-marrow biopsy showed that the metastatic tumor cells were negative for thyroglobulin on immunoperoxidase stain and the secretory product was mucicarmine-positive. We estimate that radioiodine uptake in the normal thyroid gland was less than 10% of total tumor uptake. At autopsy, the stomach was the site of the primary tumor, which had the same cellular and histochemical characteristics as the metastatic lesions in bone and liver. It is emphasized that the use of pertechnetate for screening patients with gastric adenocarcinoma may be clinically useful in the early detection of metastatic lesions.

Topics: Adenocarcinoma, Mucinous; Aged; Bone Neoplasms; Female; Humans; Iodine Radioisotopes; Liver Neoplasms; Radionuclide Imaging; Sodium Pertechnetate Tc 99m; Stomach Neoplasms; Thyroid Neoplasms

Topics: Aged; Autopsy; Female; Humans; Leiomyosarcoma; Radionuclide Imaging; Sodium Pertechnetate Tc 99m; Stomach Neoplasms; Technetium

Topics: Gastrins; Gastritis, Atrophic; Humans; Male; Middle Aged; Radionuclide Imaging; Sodium Pertechnetate Tc 99m; Stomach Neoplasms; Technetium; Tetragastrin

The authors present a case in which a patient suffered a bleeding jejunal ulcer caused by heterotopic gastric mucosa in a congenital duplication of a segment of jejunum. This is the first case diagnosed preoperatively by two different radiographic means. These lesions were shown by both pertechnetate flow and barium small bowel studies. The rarity of these entities and the modalities used for diagnosis are described.

Topics: Adult; Choristoma; Female; Gastric Mucosa; Humans; Jejunum; Peptic Ulcer Hemorrhage; Radiography; Sodium Pertechnetate Tc 99m; Stomach Neoplasms; Technetium