sodium-pertechnetate-tc-99m and Lymphoma

Warthin's tumors and oncocytomas show exceptionally good (99m)TcO4(-) (Tc) accumulation images in Tc scintigraphy. However, the mechanism of Tc accumulation in these tumors remains unclear. Sodium-iodide symporter (NIS) is a plasma membrane protein expressed in the thyroid, lactating breast, stomach and salivary glands; it facilitates uptake of I(-) and Tc. We hypothesized that Warthin's tumor cells and oncocytomas may also express NIS, which would promote uptake of Tc. We examined NIS localization and the mechanism of Tc accumulation in various salivary gland tissues.. Immunohistological localization of NIS was performed for 19 tumors from 18 patients who underwent preoperative Tc scintigraphy. Expression of mRNA for NIS in the normal salivary gland, Warthin's tumors and pleomorphic adenomas was analyzed by real-time PCR.. In normal salivary glands, striated duct cells were strongly immunostained by anti-NIS antibodies. In Warthin's tumors, eosinophilic epithelial cells exhibited positive immunostaining, but their staining was varied among the cases. Furthermore, all Tc-positive specimens were NIS-positive, and all Tc-negative specimens were NIS-negative. Real-time PCR showed that NIS mRNA expression was detectable in normal salivary glands and Warthin's tumor cells. The expression was significantly higher in normal salivary glands compared with Warthin's tumor cells and pleomorphic adenoma.. Tc-positive salivary glands expressed NIS. Our findings suggest that Tc accumulation in Warthin's tumors and oncocytomas is due to poorer Tc excretory function compared with normal salivary gland tissues, in addition to active uptake of (99m)TcO4(-) via NIS.

Topics: Adenolymphoma; Adenoma; Adenoma, Oxyphilic; Adenoma, Pleomorphic; Carcinoma, Acinar Cell; Carcinoma, Mucoepidermoid; Humans; Immunohistochemistry; Lymphoma; Radionuclide Imaging; Radiopharmaceuticals; RNA, Messenger; Salivary Gland Neoplasms; Salivary Glands; Sodium Pertechnetate Tc 99m; Symporters

Topics: Adenoma; Adolescent; Adult; Carcinoma, Squamous Cell; Gallium Radioisotopes; Humans; Lymphoma; Male; Middle Aged; Parotid Neoplasms; Radionuclide Imaging; Sodium Pertechnetate Tc 99m

This paper summarizes the clinical and diagnostic features of five reports of patients with intracerebral, non-Hodgkin's lymphoma. In three patients the brain lesion was the only evidence of lymphoma, while two patients also had concomitant systemic involvement. Four patients had diffuse histiocytic lymphoma and one had a mixed type of malignant lymphoma. In all patients, Tc-99m and Ga-67 brain scans disclosed discrete areas of increased radionuclide uptake consistent with a mass. In each case, brain blood perfusion studies were normal and brain computerized tomographic (CT) scans and cerebral angiograms produced variable nondiagnostic patterns. Craniotomies in four patients provided histologic confirmation of the non-Hodgkin's lymphoma in the areas of abnormality. The remaining patient had systemic histiocytic lymphoma with concomitant brain lesions that responded to irradiation. The combined use of the above noninvasive modalities in correlation with clinical findings may result in more accurate prebiopsy diagnoses of intracerebral lymphoma.

Topics: Adult; Aged; Brain Neoplasms; Evaluation Studies as Topic; Female; Gallium Radioisotopes; Humans; Lymphoma; Male; Middle Aged; Radionuclide Imaging; Sodium Pertechnetate Tc 99m; Technetium; Tomography, X-Ray Computed

Topics: Aged; beta 2-Microglobulin; Biopsy, Needle; Humans; Iodine Radioisotopes; Lymphoma; Male; Radioisotopes; Radionuclide Imaging; Sodium Pertechnetate Tc 99m; Technetium; Thallium; Thyroid Gland; Thyroid Neoplasms