sodium-oxybate and Cardiomyopathies

In this study, the effects of graded doses of isoproterenol (IP) on the heart were examined in untreated gerbils and in gerbils anaesthetized with gamma-hydroxybutyrate (GHB), an endogenous metabolite with energy sparing properties. We were interested in the cardioprotective potential of GHB. IP was administered intraperitoneally in doses of 0.1, 0.3. 2.5 and 10.0 mg/kg to different groups of gerbils. Half the gerbils in each treatment group received 500 mg/kg of GHB 30 min before IP, and 250 mg/kg at three subsequent 2-hour intervals. The remaining gerbils in each treatment group received saline at these time points. The animals were sacrificed after 8 hours. The accumulation of neutral fat droplets in the sarcoplasm was the most consistent effect of IP. The highest dose also produced some scattered myofibre death. The accumulation of fat in the cells could be estimated semi-quantitatively using a histochemical reaction for succinic dehydrogenase, and the volume of fat could be measured more accurately by electron microscopic morphometry. These measurements showed that IP produced a three to five-fold increase in sarcoplasmic fat volume. GHB either abolished or significantly reduced the accumulation of fat and it also completely prevented the myofibre death caused by the highest doses of IP. This cardioprotective effect of GHB was independent of its hypothermic action. Based on this experience, ultrastructural morphometry of sarcoplasmic fat appears to be a promising method for evaluating cardioprotective measures.

Topics: Animals; Cardiomyopathies; Gerbillinae; Isoproterenol; Lipids; Male; Microscopy, Electron; Mitochondria, Heart; Myocardium; Sodium Oxybate

It has been established that natrium oxybutirate, prolactin, inderal and ionol affecting different links of pathogenetic chain of stress injury, efficiently prevent structural damage to cardiomyocytes and the accumulation of 99mTc-pyrophosphate in the myocardium.

Topics: Animals; Antioxidants; Butylated Hydroxytoluene; Cardiomyopathies; Heart; Male; Prolactin; Propranolol; Psychophysiologic Disorders; Rats; Sodium Oxybate; Sodium Pertechnetate Tc 99m; Stress, Psychological

Administration of GHBA during emotional stress reduces the stress syndrome that manifests in the lesser increase of the corticosterone content in blood plasma, while the noradrenaline level in the heart remains unchanged. GHBA prevents necrosis and contracture injuries of heart muscle cells during the stress. Quantitative evaluation of GHBA protective effect on the organism exposed to stress helped determine that the preliminary administration of GHBA reduces twofold the time necessary for the appearance of eosinophilia after eosinopenia. This indicates that GHBA a powerful antistress action.

Topics: Animals; Cardiomyopathies; Corticosterone; Eosinophils; Hydroxybutyrates; Leukocyte Count; Male; Myocardium; Norepinephrine; Rats; Sodium Oxybate; Stress, Physiological