sodium-morrhuate and Necrosis

Topics: Hemangioma; Humans; Necrosis; Sclerosing Solutions; Sclerotherapy; Sodium Morrhuate

Topics: Hand; Hemangioma; Humans; Infant; Male; Necrosis; Sclerosing Solutions; Sclerotherapy; Skin Neoplasms; Sodium Morrhuate

The vessel sclerosing property of sodium morrhuate is useful in treatment of recurrent joint effusions particularly in cases of knee joint effusions. It also can be employed as an addition to surgical synovectomy. Little is known about the effects of this drug on cartilage. This study was designed to investigate the cytotoxic impact of sodium morrhuate on human chondrocytes and cartilage tissue in vitro. Primary chondrocytes from 13 patients were isolated and cultivated in three-dimensional alginate cultures. Furthermore, femoral cartilage explants of 10 patients were cultivated in vitro. Both chondrocytes and cartilage explants were exposed to mixture of sodium morrhuate and mepivacaine in different concentrations simulating chemical synovectomy. After 48 h, cell proliferation, viability, and cytotoxicity were measured. The cartilage specimens were analyzed for apoptosis by immunohistochemistry. Up to a dilution of 1:600, cells were found to be 100 % viable with a proliferation rate of 74 % compared to controls. From 1:400 onwards, a significant increase in LDH release was measured which reached at dilution of 1:200 74 % of high control, whereas histological examination showed no proof of apoptosis or necrosis in cartilage tissue. The results of this in vitro study demonstrate that the cytotoxic effects of sodium morrhuate on human chondrocytes, which lack their original extracellular matrix, manifest between dilutions of 1:500 and 1:400 and increase with higher concentrations of the drug. This effect was not found for cartilage explants, though.

Topics: Aged; Apoptosis; Cartilage, Articular; Cell Proliferation; Cell Survival; Cells, Cultured; Chondrocytes; Dose-Response Relationship, Drug; Female; Humans; Immunohistochemistry; Male; Mepivacaine; Middle Aged; Necrosis; Primary Cell Culture; Sclerosing Solutions; Sclerotherapy; Sodium Morrhuate; Time Factors; Tissue Culture Techniques

To compare the effect of Sodium Morrhuate on ECV-304 between its lipo- and normal preparation.. The ECV-304 cell line was supplemented with Sodium Morrhuate and lipo-Sodium Morrhuate in order, and the result on morphology (microscope, Giemsa Staining and electron microscope), cell activity (MTT), and flow cytometer between the two preparation were compared.. In normal preparation group, cell's edema occurred. Chromatin was like catkins. Tumefaction and degeneration of mitochondrion and endoplasmic reticulum appeared. In lipo-Sodium Morrhuate group, the membrane was creased and processus appeared. Chromatin aggregates to the membrane of nucleus was like crescent, and then broken. The apoptotic body was formed. MTT changes showed that the curve of the normal preparation group was steep and the change time was short relatively, which cues the vital cells decreased sharply. The curve of lipo-Sodium Morrhuate group was gentle and the change time was long relatively, which cues the vital cells decreased slowly. The flow cytometer showed that typical apoptosis peak appeared.. The normal preparation group shows an acute toxic effect on ECV-304 cell line, which result in a necrosis course, while lipo-Sodium Morrhuate shows a gradual releasing process, which may indicate a apoptosis course.

Topics: Animals; Apoptosis; Cell Line; Humans; Necrosis; Sclerosing Solutions; Sodium Morrhuate

Topics: Aminoglycosides; Animals; Autopsy; Carbenicillin; Disease Models, Animal; Drug Therapy, Combination; Gentamicins; Leukocyte Count; Necrosis; Osteomyelitis; Pseudomonas aeruginosa; Pseudomonas Infections; Rabbits; Radiography; Sodium Morrhuate; Time Factors