sodium-hypochlorite and Peritoneal-Neoplasms

We compared three sodium hypochlorite digestion methods and two potassium hydroxide digestion methods for detection of ferruginous bodies in human tissues. The following results were obtained. 1. Methods using sodium hypochlorite were effective for digestion of lung tissue samples, but only in part for greater omentum tissue samples. The potassium hydroxide digestion method worked well for preparation of lung and greater omentum tissue samples. 2. Results obtained by the different digestion methods were compared by Kruskal-Wallis test and Mann-Whitney U-test. No significant difference was detected in concentrations of ferruginous bodies and naked fibers in samples of lung, and tumor and normal tissues of the greater omentum among the digestion methods. 3. A more simplified procedure for sample processing with potassium hydroxide digestion was recommended for detection of ferruginous bodies in human tissues.

Topics: Humans; Hydroxides; Iron; Lung; Male; Mesothelioma; Middle Aged; Omentum; Peritoneal Neoplasms; Potassium; Potassium Compounds; Sodium Hypochlorite

Cell suspensions of chemically induced tumors (rhabdomyosarcoma) were transplanted into the peritoneal cavities of Lewis rats. In normal animals, the greater omentum was the main site of tumor growth, and transdiaphragmatic metastases to regional lymph nodes in the mediastinum were few and small. In animals during the healing phase of a chemical peritonitis, the greater omentum was fibrotic, shrunken, and inactivated. The loss of the scavenging function of the omentum was associated with wide dissemination of the tumor in the peritoneal cavity and increased access of the tumor to the lymphatic stomata on the peritoneal surface of the diaphragm. Number and size of transdiaphragmatic metastases in draining lymph nodes were greatly increased in this postinflammatory state.

Topics: Animals; Female; Fibrosis; Liver; Lymph Nodes; Lymphatic Metastasis; Mesentery; Neoplasm Metastasis; Neoplasm Transplantation; Omentum; Peritoneal Cavity; Peritoneal Neoplasms; Peritonitis; Rats; Rats, Inbred Lew; Rhabdomyosarcoma; Sodium Hypochlorite; Spleen; Tumor Cells, Cultured