sodium-hypochlorite and Melanoma

Excision with or without adjuvant cryotherapy or brachytherapy is the treatment of choice in conjunctival melanoma. Adjuvant rinsing with alcohol or sodium hypochlorite peroperatively (Dakin's solution) is used in some centers to prevent seeding of melanoma cells. The purpose of this research is to compare the cytotoxicity of sodium hypochlorite (NaOCl) with other potential cytotoxic solutions in the treatment of conjunctival melanoma.. Three uveal melanoma cell lines (OCM8, Mel285, and Mel270) and one conjunctival melanoma cell line (CM2005.1) were tested in a proliferation test (CellTiter 96 AQueous One Solution Cell Proliferation Assay, Promega, Madison, WI). The 96-well plates were coated with melanoma cells and treated with sodium hypochlorite 0.5%, sodium bicarbonate (1.4% and 8.4%), ethanol 99%, or sodium chlorite during 3, 5, or 15 minutes. Each solution was tested in several dilutions.. In all cell lines, no surviving cells were observed after treatment of 3 minutes with sodium hypochlorite. Ethanol 99% had a similar effect. A reduction of 70% of viable cells could be reached using sodium bicarbonate 1.4% or 8.4%. Water reduced the amount of viable cells by 40%.. Sodium hypochlorite is cytotoxic for melanocytic cells in vitro. Its use may reduce local seeding of tumor cells and may decrease metastasis after extirpation of an extended ocular tumour. Further in vivo evaluation of sodium hypochlorite is required.

Topics: Cell Line, Tumor; Cell Proliferation; Chlorides; Cytotoxins; Ethanol; Eye Neoplasms; Humans; Melanoma; Sodium Bicarbonate; Sodium Hypochlorite; Time Factors; Water

Two chemical agents, sodium hypochlorite 0.5% (Dakin's solution) and mercury chloride 0.1%, capable of killing seeded tumour cells, were used for washing the cornea and conjunctiva of rabbits. Both agents produced considerable superficial lesions in the cornea and conjunctiva after irrigation for 3 and 5 min, but these were restored to normal after 2 weeks.

Topics: Animals; Conjunctival Neoplasms; Humans; Melanoma; Mercuric Chloride; Mercury; Neoplasm Recurrence, Local; Rabbits; Sodium Hypochlorite

After excision of conjunctival melanomas the rate of recidivation is high. This may partly be due to local seeding of tumour cells in the excision wound, as we observed in one patient. We now use a sodiumhypochlorite solution (Dakin's solution) as tumour cell killing agent. Instead of diagnostic biopsy exfoliative cytology is performed; at surgery the tumour is not touched except for cauterisation with formaldehyde.

Topics: Conjunctival Neoplasms; Humans; Male; Melanoma; Middle Aged; Neoplasm Metastasis; Neoplasm Recurrence, Local; Sodium Hypochlorite

Topics: Animals; Carcinoma; Chlorides; Detergents; Disease Models, Animal; Disinfectants; Hydrogen Peroxide; Iodine; Melanoma; Mercury; Mice; Neoplasm Seeding; Neoplasms, Experimental; Nitrogen Mustard Compounds; Sarcoma, Experimental; Sodium Hypochlorite; Solutions; Therapeutic Irrigation