sodium-dodecyl-sulfate and Swine-Diseases

Perienteric fluid (Pf) of adult Ascaris suum was fractionated by ammonium sulfate precipitation, gel filtration or DEAE--cellulose chromatography, and sucrose density gradient centrifugation. Anti-sera (anti-EE) from pigs which were inoculated orally with fully embryonated eggs (EE) of A. suum were used in an indirect radioimmunoassay (IRIA) to determine which fractions of Pf reacted positively in the analyses at the lowest protein concentration. These fractions were considered to contain more potent antigens. Comparative IRIA were performed employing antisera (anti-Pf) produced by injecting Pf into pigs. Six out of 35 fractions reacted positively at less than or equal to 0.2 micrograms protein when anti-EE was used in the IRIA. Twenty-two out of 35 fractions reacted positively at less than or equal to 0.2 micrograms protein when anti-Pf was used. Five of the 6 fractions reacting positively with anti-EE also reacted with anti-Pf. A 76000 dalton component appears to be one of the major proteins in the fractions which react positively with anti-EE while components from 10000-138000 daltons were present in the various fractions reacting positively with anti-Pf at less than or equal to 0.2 micrograms protein.

Topics: Animals; Antibodies; Antigens; Ascariasis; Ascaris; Centrifugation, Density Gradient; Chromatography, DEAE-Cellulose; Electrophoresis, Polyacrylamide Gel; Radioimmunoassay; Sodium Dodecyl Sulfate; Swine; Swine Diseases