sodium-dodecyl-sulfate and Ovarian-Neoplasms

Application of novel technology to clinical samples requires optimization of procedures. Reverse phase protein lysate arrays use femtomolar quantities of tissue lysate from clinical samples with which to profile biochemical events happening in the tumor. We analyzed the effects of different tissue solubilization buffers on frozen ovarian tumor samples in order to identify the system with the best signal intensity dynamic range, reproducibility, tissue solubility, and signal consistency. A modified RIPA-like buffer supplemented with DTT and SDS was deemed optimal.

Topics: Buffers; Dithiothreitol; Female; Humans; Neoplasm Proteins; Ovarian Neoplasms; Protein Array Analysis; Proteomics; Reproducibility of Results; Sodium Dodecyl Sulfate; Solubility

Human polymorphic epithelial mucin is a high-molecular-mass glycoprotein that associates to provide protection to the epithelial-cell surface and may afford the malignant cell a selective advantage for growth. The scanning-tunnelling-microscopy micrographs obtained in the present study identify the purified human ovarian-carcinoma polymorphic epithelial mucin glycoproteins as rod-shaped molecules of mixed length. The dimensions of the individual molecules range from 25 to 45 nm in length and are 3-4 nm in width. The images further suggest that lateral association of the rods occurs.

Topics: Animals; Antibodies, Monoclonal; Ascitic Fluid; Blotting, Western; Electrophoresis, Polyacrylamide Gel; Female; Humans; Membrane Glycoproteins; Mice; Microscopy, Scanning Tunneling; Molecular Structure; Mucin-1; Mucins; Ovarian Neoplasms; Sodium Dodecyl Sulfate

Topics: Amino Acids; Ascitic Fluid; Disulfides; Electrophoresis, Polyacrylamide Gel; Female; Haptoglobins; Humans; Macromolecular Substances; Mercaptoethanol; Molecular Weight; Ovarian Neoplasms; Oxidation-Reduction; Peptides; Protein Conformation; Sodium Dodecyl Sulfate; Ultracentrifugation