sodium-dodecyl-sulfate and Leukopenia

Spectrophotometric and gas-liquid chromatographic analyses on the carbohydrate moiety of tryptic erythrocyte glycopeptides from persons with Tn-syndrome reveal a selective lowering of the galactose and sialic acid content, the degree being dependent on the percentage of polyagglutinable cells. Alkaline borohydride specifically releases N-acetylgalactosaminitol, and the amount is correlated to the percentage of pathological acetylgalactosaminitol, and the amount is correlated to the percentage of pathological erythrocytes. It is concluded that the alkali-labile carbohydrate chains of Tn-polyagglutinable red cells solely consist of N-acetylgalactosamine linked to serine or threonine. Experiments with heterophile agglutinins whose specificity is known are in line with the above-mentioned results. As judged from SDS-polyacrylamide gel electrophoresis the three major membrane glycoproteins are affected to a different extent by the defect.

Topics: Anemia, Hemolytic; Antibodies, Heterophile; Blood Glucose; Blood Protein Electrophoresis; Cell Membrane; Chromatography, Gas; Electrophoresis, Polyacrylamide Gel; Erythrocytes; Glycopeptides; Hemagglutination; Hemagglutination Tests; Hexoses; Humans; Leukopenia; Molecular Weight; Sialic Acids; Sodium Dodecyl Sulfate; Syndrome; Thrombocytopenia; Trypsin