sodium-dodecyl-sulfate and Keratosis

Hand eczema (HE) is a multifactorial disease, comprising different aetiological conditions and different morphologies. There are two aetiologically distinct groups of HE recognised: exogenous, such as contact dermatitis (allergic and/or irritant HE) and endogenous, such as the classic hyperkeratotic HE. Differences in the skin barrier properties of these two conditions could theoretically be expected.. To examine whether differences exist in the lipid profile and the susceptibility of the stratum corneum (SC) in patients with allergic/irritant HE and those with hyperkeratotic HE.. Using cyanoacrylate, SC samples were taken from 23 patients with allergic/irritant HE and 15 with hyperkeratotic HE for lipid analysis by high-performance thin-layer chromatography (HPTLC). Samples were also taken from adjacent, unaffected skin. Severity of HE was assessed by the Hand Eczema Severity Index (HECSI), and skin barrier susceptibility was assessed by measuring transepidermal water loss (TEWL) after a 24-hour patch test with sodium lauryl sulfate (SLS).. No statistically significant difference was found between groups for the lipid analysis or for skin susceptibility to SLS. We found a significantly higher HECSI score for hyperkeratotic HE compared with irritant or allergic HE (P = 0.02).. There appears to be no difference in skin barrier between allergic/irritant HE (exogenous eczema) and hyperkeratotic HE (endogenous eczema) with regard to SC lipids or susceptibility to SLS.

Topics: Adult; Aged; Ceramides; Chromatography, High Pressure Liquid; Eczema; Epidermis; Female; Hand Dermatoses; Humans; Irritants; Keratosis; Male; Middle Aged; Patch Tests; Skin; Sodium Dodecyl Sulfate; Surface-Active Agents; Water Loss, Insensible; Young Adult

Topics: Animals; Cell Line; Dermatitis; Disease Models, Animal; Epidermis; Gene Expression Regulation; Humans; Kallikreins; Keratinocytes; Keratosis; Melanosis; Mice; Mice, Inbred C57BL; Mice, Knockout; Nerve Growth Factor; Real-Time Polymerase Chain Reaction; Receptors, Nerve Growth Factor; RNA Interference; RNA, Messenger; Sodium Dodecyl Sulfate; Transfection

The formation of lacunae and acantholysis as well as dyskeratosis are characteristic features of Hailey-Hailey disease (HHD) and Darier's disease (DD). Matrix metalloproteinases (MMPs) and their inhibitors like tissue inhibitors of metalloproteinases (TIMPs) have been thought to play major roles in the tissue metabolism.. The aim of this study was to investigate the role of MMP-9 and TIMP-1 in HHD and DD.. We examined localizations of these two molecules by immunostaining using specific monoclonal antibodies.. MMP-9 was positively stained in dyskeratotic or detaching cells around lacunae in HHD and DD. TIMP-1 showed a positive staining pattern throughout the epidermis.. MMP-9 might be involved in the pathophysiological process of HHD and DD in the presence of TIMP-1.

Topics: Acantholysis; Antibodies, Monoclonal; Blotting, Western; Cell Adhesion; Cells, Cultured; Collagenases; Coloring Agents; Darier Disease; Electrophoresis, Polyacrylamide Gel; Epidermis; Glycoproteins; Humans; Immunohistochemistry; Keratinocytes; Keratosis; Matrix Metalloproteinase 9; Matrix Metalloproteinase Inhibitors; Pemphigus, Benign Familial; Protease Inhibitors; Sodium Dodecyl Sulfate; Tissue Inhibitor of Metalloproteinases

Topics: Animals; Basement Membrane; Blister; Cytoplasm; Edema; Female; Inflammation; Keratosis; Lipid Metabolism; Microscopy, Electron; Rats; Ribosomes; Skin; Sodium Dodecyl Sulfate