sodium-dodecyl-sulfate and Hypertension

The (Na+,K+)-ATPase activity from the kidney cortex of the Milan hypertensive rat strain (MHS) and the corresponding normotensive control (MNS) was measured both in active solubilized enzyme preparations and in isolated basolateral membrane vesicles. Kinetic analysis of the purified enzyme showed that the Vmax value was significantly higher in MHS rats. The difference between MHS and MNS was not linked to a different number of sodium pumps, but was related to the molecular activity of the enzyme. Using basolateral membrane vesicles, an increased ATP-dependent ouabain-sensitive sodium transport was also demonstrated in MHS rats. These results support the hypothesis that a higher tubular sodium reabsorption may be involved in the pathogenesis of hypertension in this rat strain.

Topics: Animals; Cell Membrane; Hypertension; In Vitro Techniques; Kidney Cortex; Kinetics; Ouabain; Potassium; Rats; Rats, Mutant Strains; Sodium; Sodium Dodecyl Sulfate; Sodium-Potassium-Exchanging ATPase

In patients with essential hypertension, an additional plasma protein with an apparent molecular weight of 15,000 has been observed by high resolution sodium dodecyl sulphate polyacrylamide gel electrophoresis in the presence of beta-mercaptoethanol. Gel filtration on Sephacryl S-300 superfine revealed a native molecule with a molecular weight of 105,000. After reduction of the disulfide bridges with beta-mercaptoethanol the native molecule is dissociated into two subunits of 45,000 and one of 15,000. The native plasma protein has been purified by a combination of gel filtration on Sephacryl S-300, affinity chromatography on Concanavalin A Sepharose and anion exchange chromatography on DEAE-Sepharose. This protein has been referred to as "hypertension associated protein" (HAP).

Topics: Blood Proteins; Chromatography, Gel; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel; Humans; Hypertension; Molecular Weight; Sodium Dodecyl Sulfate

In patients with essential hypertension, a variation in the plasma proteins was revealed by high-resolution sodium dodecyl sulphate polyacrylamide gel electrophoresis. This variation in plasma protein was not found in patients with hypertension secondary to renovascular or renal parenchymal disease.

Topics: Adult; Blood Proteins; Electrophoresis, Polyacrylamide Gel; Humans; Hypertension; Middle Aged; Sodium Dodecyl Sulfate

Topics: Adolescent; Adult; Aged; Child; Diabetes Mellitus; Electrophoresis, Polyacrylamide Gel; Female; Humans; Hypertension; Kidney Diseases; Kidney Glomerulus; Kidney Tubules; Male; Methods; Middle Aged; Molecular Weight; Nephrotic Syndrome; Proteinuria; Sodium Dodecyl Sulfate; Urine

Topics: Animals; Cellulose; Chromatography, Gel; Diabetes Mellitus; Dialysis; Electrophoresis; Electrophoresis, Polyacrylamide Gel; Evaluation Studies as Topic; Horses; Humans; Hydrogen-Ion Concentration; Hypertension; Immunoelectrophoresis; Kidney Diseases; Methods; Molecular Weight; Proteinuria; Sodium Dodecyl Sulfate; Time Factors; Ultrafiltration