sodium-dodecyl-sulfate and Fetal-Membranes--Premature-Rupture

Amniorrhexis complicates pregnancies if it occurs in a preterm pregnancy or remote from the onset of labor in a term pregnancy. There are different collagen types (I-V) that create the extracellular matrix of the amnion. Collagenases specific to these collagen types, with the exception of type V collagen, are found in human amniotic fluid, fibroblasts, polymorphonuclear leukocytes and bacteria. Type V collagen is a major component of the amniotic basement membrane and is responsible for maintaining a barrier to bacteria and to the loss of amniotic fluid. We sought to find evidence of type V collagenolytic activity in human amniotic fluid obtained from pregnancies in different clinical states.

Topics: Amniocentesis; Amnion; Amniotic Fluid; Autoradiography; Bacteria; Basement Membrane; Collagen; Collagenases; Electrophoresis, Polyacrylamide Gel; Extracellular Matrix Proteins; Female; Fetal Membranes, Premature Rupture; Fetal Organ Maturity; Fibroblasts; Genetic Testing; Humans; Lung; Matrix Metalloproteinase 8; Microbial Collagenase; Neutrophils; Obstetric Labor, Premature; Pilot Projects; Placenta; Pregnancy; Sodium Dodecyl Sulfate; Surface-Active Agents