sodium-dodecyl-sulfate and Dysentery--Bacillary

The authors evaluated the immunological results of enteral and subcutaneous injection of detergent Sonne dysentery vaccine to volunteers (changes in homo- and heterologous antibodies in the blood and saliva recorded by means of common indirect hemagglutination test and that modified according to Coombs' test, and IgG, IgM, and IgA concentration recording. Both immunization methods induced specific antibody formation: enteral vaccination caused chiefly the accumulation of secretory antibodies of IgA class, and subcutaneous -- of serum antibodies belonging to IgG class. The supposed mechanisms determining the capacity of enteral administration of the antigen to provoke an immune response different from that caused by its subcutaneous injection are discussed.

Topics: Administration, Oral; Antibodies, Bacterial; Bacterial Vaccines; Clinical Trials as Topic; Drug Evaluation; Dysentery, Bacillary; Feces; Humans; Immunoglobulin A; Immunoglobulin A, Secretory; Immunoglobulin G; Immunoglobulin M; Injections, Subcutaneous; Saliva; Shigella sonnei; Sodium Dodecyl Sulfate; Time Factors; Vaccination

Chemical (detergent) antigens administered intraintestinally or intravenously to rabbits were applied to reproduce postvaccinal immunity to experimental shigellosis. There was noted an increase of the antibody level in the serum, an intensification of its preventive proerties, and the absence of dilatation of the isolated loops of the small intestine after the administration of the corresponding dysentery culture. Oral immunization of rabbits with the detergent antigen sorbed on ionexchange resin also led to immunity.

Topics: Administration, Oral; Animals; Antibodies, Bacterial; Antigens; Detergents; Dysentery, Bacillary; Hemagglutination Tests; Injections; Injections, Intravenous; Intestine, Small; Ligation; Rabbits; Shigella sonnei; Sodium Dodecyl Sulfate; Vaccines