sodium-dodecyl-sulfate and Carcinoma--Intraductal--Noninfiltrating

Although the hamster pancreas does not express A, B or H blood group antigens, all hamster pancreatic ductal adenocarcinomas induced by treatment with N-nitrosobis(2-oxopropyl)amine express blood group-A antigen. Thus, the acquisition of blood group-A antigen expression in this system is a cancer-associated alteration. We have purified three major blood group-A antigen bearing glycoproteins (gp120, gp135 and gp150) from hamster pancreatic cancer cell membrane preparations using affinity chromatography on DBA (Dolichos biflorus) agglutinin-agarose. When assayed by immunoblotting, gp120 and gp135 showed strong blood group-A reactivity, which was removed by treating membrane samples with peptide-N-glycosidase F. Blood group-A reactivity was unchanged by treatment of the membrane fractions with endoglycosidases F and H. In addition, these two glycoproteins bearing blood group-A antigen also bound L-PHA (Phaseolus vulgaris leucoagglutinin). These results demonstrate that gp120 and gp135 express blood group-A antigen on Asn-linked multi-antennary complex type glycan structures. The gp150 showed weak blood group-A expression. This is the first demonstration of the neoexpression of cancer-associated blood group-A determinants which reside on Asn-linked glycan structures.

Topics: ABO Blood-Group System; Animals; Antigens, Neoplasm; Carcinogens; Carcinoma, Intraductal, Noninfiltrating; Cricetinae; Electrophoresis, Polyacrylamide Gel; Epitopes; Glycoside Hydrolases; Immunoblotting; Lectins; Membrane Glycoproteins; Nitrosamines; Oligosaccharides; Pancreas; Pancreatic Neoplasms; Plant Lectins; Sodium Dodecyl Sulfate; Tumor Cells, Cultured