sodium-dodecyl-sulfate and Anaphylaxis

Patients allergic to garlic often present dermatitis, rhinitis, asthma, and urticaria after ingestion of garlic, contact with garlic, or exposure to garlic dust. Garlic-related anaphylaxis is rare, and the impact of heating on garlic allergens is not very clear. We report a case of anaphylaxis induced by ingestion of raw rather than cooked garlic with manifestations different from previous reports, and we hypothesized that heating could reduce the allergenicity of garlic. Serum total immunoglobulin E (IgE) and specific IgE were tested using the Phadia CAP System FEIA (Phadia, Uppsala, Sweden). Protein extracts from raw and cooked garlic were analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot. Serum-specific IgE for garlic was 8.16 kUA/L. IgE banding proteins could only be detected in raw garlic extract, because allergens in garlic were mostly degraded into small fragments after heating, as shown in SDS-PAGE profile. In conclusion, raw garlic could induce life-threatening anaphylaxis. However, most of its allergens are heat labile, and patients allergic to garlic might tolerate the cooked one well.

Topics: Adult; Allergens; Anaphylaxis; Blotting, Western; Cooking; Electrophoresis, Polyacrylamide Gel; Food Hypersensitivity; Garlic; Humans; Immunoglobulin E; Male; Sodium Dodecyl Sulfate

Topics: Adult; Anaphylaxis; Angioedema; Beer; Double-Blind Method; Electrophoresis, Polyacrylamide Gel; Female; Food Hypersensitivity; Humans; Male; Placebos; Pruritus; Sodium Dodecyl Sulfate

The steady increase in anaphylactic reactions to latex medical gloves has raised increasing awareness in the medical community. Even gloves claimed to be hypo-allergenic still may contain substantial amounts of IgE-binding proteins. We have studied non-powdered latex and synthetic examination glove extracts for their allergenicity using the immunoblot technique. Protein levels varied considerably among glove extracts and the amount did not always correlate with the presence of allergenic proteins. IgE binding proteins were found in 2 of the 7 powder-free latex glove brands. Synthetic glove extracts did not contain allergens. The study demonstrates that immunoblot analysis is a useful technique in order to select gloves with a minimal risk to raise IgE antibodies.

Topics: Allergens; Anaphylaxis; Electrophoresis, Polyacrylamide Gel; Gloves, Surgical; Humans; Immunoblotting; Immunoglobulin E; Latex; Occupational Diseases; Powders; Protein Binding; Proteins; Rubber; Sodium Dodecyl Sulfate

In active anaphylactic shock of rats pretreated with Bordetella pertussis vaccine, both plasma thrombin clotting time and the amount of antigenically active fibrinogen degradation products in the serum were increased. The formation of clottable fibrinogen fragments was shown by SDS polyacrylamide gel electrophoresis of thrombin-induced clots. When plasma of rats pretreated with 125I rat fibrinogen and then subjected to anaphylaxis was submitted to SDS polyacrylamide gel electrophoresis, fibrinogen-split products were also detected. Fibrinogen degradation results from the proteolytic effect of an activated fibrinolytic enzyme.

Topics: Anaphylaxis; Animals; Antigens; Blood Coagulation; Blood Coagulation Tests; Electrophoresis, Disc; Fibrin Fibrinogen Degradation Products; Fibrinogen; Male; Pertussis Vaccine; Rats; Sodium Dodecyl Sulfate; Thrombin; Trypsin