sodium-cyanate and Body-Weight

In order to evaluate the toxic effects of Sodium Cyanate (NaOCN), it was orally administered to growing mice at sea level (SL-CN) and to mice chronically exposed to intermittent hypobaric hypoxia (IHH-CN). The effects on body weight, in-vivo O2 consumption (VO2) and the respiratory function of liver mitochondria were evaluated. At sea level, the animals on cyanate lost weight in contrast with the controls that gained weight. When exposed to IHH, the controls lost weight and the animals on cyanate regained weight. After 2 months observation the weights of the IHH-CN and IHH-C were similar. The VO2 after one month of treatment was similar in the SL-C and in the SL-CN but it was lower in the IHH-CN when compared with IHH-C. The substrate-stimulated respiration of isolated liver mitochondria (ST4) was not affected by NaOCN, but the ADP-stimulated respiration (ST3) was reduced. The ratio ST3/ST4 (RCR) was also lower. These changes were present in both SL and in IHH and were much larger after three months of treatment. The toxic effects of chronic administration of NaOCN are discussed.

Topics: Administration, Oral; Animals; Atmospheric Pressure; Body Weight; Cyanates; Hypoxia; Male; Mice; Mitochondria, Liver; Oxygen Consumption

Sodium cyanate injected IP at a dose level of 200 or 250 mg/kg caused a 90% or greater inhibition of the incorporation of [3H]thymidine into DNA of B16 melanoma transplanted SC in mice. Despite the inhibitory effect of sodium cyanate on precursor incorporation into DNA, no significant effect on host survival was observed when sodium cyanate was administered as a single agent in the diet, in drinking water, or by IP injection to mice that had received IP transplants of B16 melanoma. The action of melphalan and 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU) in prolonging the survival time of melanoma-bearing mice was not enhanced by combined treatment with sodium cyanate. However, combined injections of sodium cyanate and 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) increased the survival of tumor-bearing mice significantly more than injections of BCNU alone at a lower dose than the maximum tolerated one. These data and other studies suggest that B16 melanoma may be less responsive to the action of sodium cyanate than are murine leukemic cells or rat hepatomas.

Topics: Animals; Antineoplastic Agents; Antineoplastic Combined Chemotherapy Protocols; Body Weight; Carmustine; Cyanates; Eating; Female; Lomustine; Melanoma; Mice; Mice, Inbred C57BL; Thymidine

Topics: Animals; Blood Gas Analysis; Body Weight; Cardiac Output; Chemical Phenomena; Chemistry, Physical; Cyanates; Hydrogen-Ion Concentration; Hypoxia; Lung; Male; Oxygen; Oxygen Consumption; Rats; Rats, Inbred Strains

Topics: Administration, Oral; Adolescent; Adult; Anemia, Sickle Cell; Bilirubin; Body Weight; Carbamates; Chemical Phenomena; Chemistry; Child; Cyanates; Dose-Response Relationship, Drug; Drug Evaluation; Erythrocyte Count; Female; Gastrointestinal Diseases; Hemoglobins; Hemolysis; Humans; Male; Middle Aged; Reticulocytes; Retrospective Studies; Sodium